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《Chongqing Medicine》 2017-05
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The effect of astragaloside on TGF-β1,SMAD2/3,and α-SMA expression in the kidney tissues of diabetic KKAy mice

Wang Yaning;Liu Yunqi;Gao Jinxiang;Lin Chao;Liu Naiguo;Department of Nephrology,the Affiliated Hospital of Binzhou Medical University;Department of Bone and Joint Surgery,the Affiliated Hospital of Binzhou Medical University;Department of Clinical and Medicine Lab,the Affiliated Hospital of Binzhou Medical University;  
Objective To study the effect of astragaloside on TGF-β1,SMAD2/3,andα-SMA expression in the kidney tissue of diabetic KKAy mice,and evaluate its potential role in renal interstitial fibrosis.Methods 20 type 2diabetic KKAy mice were randomly divided into model group and astragaloside group,while 10 male C57BL/6Jmice were selected as the control.Astragaloside at 40mg·kg-1·d-1 was given when the KKAy mice fed with high-fat diet to 14 weeks old.The mice in the control and model group received normal saline at 40mg·kg-1·d-1.Blood glucose meter was used to detect the blood glucose value of each mice at16 th,20th and 24 th week.The mice were killed at 24 weeks old and the kidney tissue samples were collected.Pathology morphological changes were observed.Results(1)blood glucose value:cmpared with the control group,the blood glucose value of KKAy mice at 14 week increased significantly,and that of model group also increased significantly at 16 th,20th and 24 th week(P0.05);the blood glucose value of astragaloside group decreased compared with control group(P0.05).(2)Morphology of kidney:in the control group,the glomerular and tubular had clear structure,there was no renal interstitial fibrosis;in the model group,the renal glomerular mesangial matrix had broaden,mesangial cell had increased,renal tubular epithelial cell cytoplasm showed vacuole degeneration,renal interstitial inflammatory cell had increased.In astragaloside group,there were few renal tubular epithelial cell cytoplasm,and there was no obvious fibrosis.(3)TGF-β1,SMAD2/3,andα-SMA expression levels of the kidney issuse:compared with control group,mice in model group up-regulated TGF-β1,SMAD2/3andα-SMA expression(P0.05).TGF-β1,SMAD2/3,andα-SMA expression levels in astragaloside group were significantly lower than those in the model group(P0.05).There was few phosphorylated SMAD2/3expression in renal tubular and glomerular nuclei,while that of model group increased(P0.01),and compared with model group,that of the astragaloside group decreased(P0.05).Conclusion Astragaloside can delay the renal fibrosis process in diabetic mice by influencing the TGF-β/SMADS signaling pathway and down-regulating TGF-β1andα-SMA expression,thus to relieve renal fibrosis in diabetic mice.
【CateGory Index】: R285.5
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 HE Feng1,YU Lu2,TONG Jun-rong3,LUO Zheng-mao3,ZHU Qi-zhi3,WANG Yin3,ZHANG Jian-lin3 1Graduate College of Southern Medical University,Guangzhou 510515,China; 2305 Hospital of PLA,Beijing 100017,China; 3Department of Nephrology,Guangzhou General Hospital of Guangzhou Command,Guangzhou 510010,China;Role of integrin-linked kinase in renal tubular epithelial-mesenchymal transition and the regulatory effect of urokinae on its expression in mice with obstructive nephropathy[J];南方医科大学学报;2009-05
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 Li Xiaobo;Peng Bangya;Yang Jiangquan;Chen Yang;Department of Diagnostics,Zhuhai Campus of Zunyi Medical University;Department of Biochemistry and Molecular Biology,Zunyi Medical University;Department of Pharmacy,Zhuhai Campus of Zunyi Medical University;;Effects of Persicae Semen and safflower on the expressions of ILK,E-cad,FN and α-SMA in the renal tissue of unilateral ureteral obstruction rats[J];遵义医学院学报;2017-02
2 Wang Yaning;Liu Yunqi;Gao Jinxiang;Lin Chao;Liu Naiguo;Department of Nephrology,the Affiliated Hospital of Binzhou Medical University;Department of Bone and Joint Surgery,the Affiliated Hospital of Binzhou Medical University;Department of Clinical and Medicine Lab,the Affiliated Hospital of Binzhou Medical University;;The effect of astragaloside on TGF-β1,SMAD2/3,and α-SMA expression in the kidney tissues of diabetic KKAy mice[J];重庆医学;2017-05
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4 Yan Li-jing1,Tian He2,Guo Min2 1 Microscope Centre,Liaoning Medical University,Jinzhou 121001,Liaoning Province,China;2 Department of Histology and Embryology,Liaoning Medical University,Jinzhou 121001,Liaoning Province,China;Fibronectin and integrin alpha 5 expression during the development of mouse renal tubule[J];中国组织工程研究与临床康复;2011-33
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 WANG Yin1, MA Li1, TONG Jun-rong2, LUO Zheng-mao2, HE Feng2 1Institute of Molecular Immunology, School of Biotechnology, Southern Medical University, Guangzhou 510515, China; 2Deparment of Nephrology, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, China;Effect of urokinase on renal interstitial inflammation in rats with chronic cyclosporine A nephropathy and its possible mechanism[J];南方医科大学学报;2008-05
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