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《Chongqing Medicine》 2017-06
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10058-F4 could cause cervical cancer cells apoptosis

Chen Ying;Ning Yang;Huang Lei;Li Li;Wang Yulan;Li Rui;Tian Xun;Li Hemei;Zhang Qinghua;Department of Gynaecology and Obstetrics,Wuhan Central Hospital Affiliated to Huazhong University of Science and Technology;  
Objective To explore the role and mechanism of 10058-F4 on cervical cancer SaSki cells in the apoptosis.Methods The CCK8 assay was used to confirmed 10058-F4 inhibited the proliferation of CaSki cells in a dose-dependent and time dependent manner.Cell apoptosis and the ROS production was analyzed by flow cytometry.The mRNA and protein levels after cells treated with 10058-F4 were detected by real time PCR and Western blot.Results 10058-F4,an inhibitor of c-MYC,could inhibit the proliferation of CaSki cells in a dose-dependent and time dependent manner.Cells grow much slower after treated with 10058-F4for48 h,and IC50 was 122μmmol/L.In addition,cells which were treated with 10058-F4 for 48hshow apparent apoptosis and the level of cleaved-PARP confirm that.We also found that 10058-F4 reduced the expression levels of NDUFS1,SDHA,UQCRC2,COXIV and OSCP and caused excess production of ROS.Conclusion 10058-F4 leads to the decreased expression level of mitochondrial complexes,increased production of ROS,then induce apoptosis.
【Fund】: 武汉市卫生局临床医学科研项目资助(WX11B05)
【CateGory Index】: R737.33
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Chinese Journal Full-text Database 1 Hits
1 Gao Kun,Zhang Caihong,Chen Xinqiu,Li Li(Department of Gynecological Oncology,Affiliated Tumor Hospital,Guangxi Medical University,Nanning,Guangxi 530021,China);Observation on short term effects of concurrent hyperthermia and radiochemotherapy in treatment for medium-term and advanced cervical cancer[J];重庆医学;2013-07
Chinese Journal Full-text Database 6 Hits
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