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《Chongqing Medicine》 2017-06
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10058-F4 could cause cervical cancer cells apoptosis

Chen Ying;Ning Yang;Huang Lei;Li Li;Wang Yulan;Li Rui;Tian Xun;Li Hemei;Zhang Qinghua;Department of Gynaecology and Obstetrics,Wuhan Central Hospital Affiliated to Huazhong University of Science and Technology;  
Objective To explore the role and mechanism of 10058-F4 on cervical cancer SaSki cells in the apoptosis.Methods The CCK8 assay was used to confirmed 10058-F4 inhibited the proliferation of CaSki cells in a dose-dependent and time dependent manner.Cell apoptosis and the ROS production was analyzed by flow cytometry.The mRNA and protein levels after cells treated with 10058-F4 were detected by real time PCR and Western blot.Results 10058-F4,an inhibitor of c-MYC,could inhibit the proliferation of CaSki cells in a dose-dependent and time dependent manner.Cells grow much slower after treated with 10058-F4for48 h,and IC50 was 122μmmol/L.In addition,cells which were treated with 10058-F4 for 48hshow apparent apoptosis and the level of cleaved-PARP confirm that.We also found that 10058-F4 reduced the expression levels of NDUFS1,SDHA,UQCRC2,COXIV and OSCP and caused excess production of ROS.Conclusion 10058-F4 leads to the decreased expression level of mitochondrial complexes,increased production of ROS,then induce apoptosis.
【Fund】: 武汉市卫生局临床医学科研项目资助(WX11B05)
【CateGory Index】: R737.33
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Gao Kun,Zhang Caihong,Chen Xinqiu,Li Li(Department of Gynecological Oncology,Affiliated Tumor Hospital,Guangxi Medical University,Nanning,Guangxi 530021,China);Observation on short term effects of concurrent hyperthermia and radiochemotherapy in treatment for medium-term and advanced cervical cancer[J];重庆医学;2013-07
【Co-citations】
Chinese Journal Full-text Database 6 Hits
1 Zhao Qingwen;Diao Jianfeng;Wen Qing;Song Li;Ren Yun;Department of Medical Oncology,the Second People's Hospital of Datong;;Short-term effect of hyperthermia combined with concurrent radiochemotherapy on middle stage and advanced cervical cancer[J];肿瘤研究与临床;2016-08
2 YU Qing-qing;TANG Rong-jun;LOU Jun;YU Yu-feng;HE Ling-hong;Department of Radiotherapy,Hangzhou Tumor Hospital;Radiation Physics Room,Hangzhou Tumor Hospital;Department of Ultrasound,Hangzhou Tumor Hospital;;Application value of simultaneous thermal chemoradiotherapy for advanced cervical cancer[J];中国妇幼健康研究;2015-04
3 TANG Ji-ying;CAI Xiao-jun;CHEN Ping;PAN Dong-feng;Department of Oncology,Renmin Hospital,Hubei University of Medicine;;Intensity Modulated Radiotherapy Combined with Concurrent Hyperthermia,Chemotherapy for Recurrent Cervical Cancer after Surgical Therapy[J];湖北医药学院学报;2015-01
4 YAN Xiang-yong;LIU Wen-chao;YAN Zhong-sheng;MA Ji;Department of Clinical Oncology,h e Xijing Hospital,Fourth Military Medical University;Department of Integrative Medicine,Lanzhou Military Region General Hospital;;Ei cacy and Safety Radio-chemotherapy Combined with h ermotherapy for Cervical Cancer: A Meta-Analysis[J];中国循证医学杂志;2014-06
5 MA Xiao-ping;YANG Yong-xiu;GE Yan;The First Clinical Medicine College of Lanzhou University;Department of Obstetrics and Gynecology,the First Affiliated Hospital of Lanzhou University;;HCRT for Adva nced Cervical Carcinoma:A Meta-analysis[J];国际妇产科学杂志;2014-03
6 MAO Hong-hua;ZHONG Yan;Department of Hematology Oncology,Shaoyang Central Hospital;;Clincal study on endogenetic field tumor hyperthermia combined with Chemotherapy and Radiotherapy in the treatment of advanced Cervical Cancer[J];中国肿瘤临床与康复;2014-05
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