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《Chongqing Medicine》 2017-11
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Placental Grb10 expression analysis for evaluation of security for blastocyst vitrification

Lin Dianliang;Quan Song;Kang Yuefan;Yi Jinsong;Yu Aili;Lin Yuan;Human Assisted Reproductive Research Unit,Fujian Provincial Maternity and Children′s Hospital of Fujian Medical University;Center for Reproductive Medicine,Department of Obstetrics and Gynecology,Nanfang Hospital,Southern Medical University;  
Objective To analyze the expression of the placenta Grb10 from women conceived by transferred thawed blastocyst,and to evaluate the security of blastocysts vitrification.Methods A cross-sectional study was performed in the Department of Obstetrics and Gynecology of Fujian Provincial Maternity and Children′s Hospital from January 2012 to May 2014,50 women conceived by transferring thawing blastocyst and 50 natural pregnancy control women were enrolled in this study.The expression of Grb10 protein was detected by immunohistochemistry and Western blot,and the expression of Grb10 mRNA was detected by Realtime PCR method.Results Comparison of two cases of gestational age,gestational age,fetal sex,fetal body weight,body length,head circumference,abdominal circumference,there were no significant differences(P0.05),comparison of placental area,placental weight,the difference was statistically significant(P0.05).Real-time PCR and Western blot results showed that,there was no significant difference in the expression of Grb10 mRNA and protein between the two groups(P0.05).Conclusion Blastocysts vitrification may increase the area and quality of delivery of placenta,however,there was no significant change in the expression of Grb10 in placenta.
【Fund】: 福建省自然科学基金(2014J01277);; 福建省卫生系统中青年骨干人才培养项目(2013-ZQN-JC-7);; 福建省妇幼保健院科研基金(闽妇幼13-08 13-09 10-01)
【CateGory Index】: R714.8
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