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《Chongqing Medicine》 2017-12
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Effect of bone marrow mesenchymal stem cell transplantation on silicosis fibrosis in rats

Chen Zhijun;Huang Huixia;Xie Ying;Li Donghong;Li Zhimin;Zhang Jianjie;Department of Occupational Disease,Shenzhen Hospital for Occupational Disease Prevention and Treament;  
Objective To study the effects of bone mesenchymal stem cells(BMSCs)transplantation on pulmonary tissue fibrosis in silicosis rats.Methods Forty-two healthy female SD rats were randomly divided into 4groups:group A,B,C and D.The group A,B and C received intratracheal injection of silica crystals(SiO2)to establish silicosis mouse models,while the group D received the infusion of physiological saline.BMSCs were injected by the tail vein at 6hafter modeling or on 42 din the group B and C.Six rats in each of group A,B,and D were sacrificed on 14 d,and other 6rats in each group were sacrificed on 56 day.The morphological changes of the lung tissues were observed by HE staining;the content of hydroxyproline(Hyp)in the lung tissue homogenate was detected by ELISA.Results The lung tissue pathological changes in the group A were dominated by acute inflammatory response on 14 dafter model construction,and fibrosis reaction on 56d;the pathological changes in the group B and C were lighter than those in the group A;while no obvious abnormality was found in the group D.The Hyp level of pulmonary tissue in the group A,B and C was significantly increased compared with the group D(P=0.000);which on 14 dafter BMSCs treatment in the group B and C was significantly decreased compared with the group A(P=0.000,P=0.035),and which on 56 dafter treatment in the group B was still significantly decreased(P=0.016).Conclusion No matter in the pathological stages of pulmonary alveolitis and fibrosis,BMSCs transplantation may postpone the pathological process of pulmonary fibrosis in silicosis rats.
【Fund】: 国家职业病临床重点专科建设项目(WY2011873);; 深圳市科技计划项目(JCYJ20140414110951762)
【CateGory Index】: R135.2
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