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《Chongqing Medicine》 2017-13
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Comparison among 3 kinds of culture substrates of odontogenic induced pluripotent stem cells

Tan Xiaobing;Liu Jia;Guo Yu;Xu Jingshu;Dai Qingyuan;Department of Endodontics,Yunnan Provincial First People′s Hospital/Kunming Univerty of science and Technology Affiliated Hospital;Department of Cardiology,First Affiliated Hospital of Kunming Medical University;  
Objective To comparatively study the characteristics of 3kinds of culture substrates of human odontogenic induced pluripotent stem cells(iPSCs).Methods The human odontogenic iPSCs were cultured by 3kinds of substrates:mouse embryonic fibroblasts(MEF),matrigel and recombinant human vitronectin(VTN-N).The iPSCs growth situation was compared among three groups.Results The preparation time of these 3kinds of substrates was 14,3,1hlespectively,and,the difference was statistically significant(P0.05).The iPSCs reprogramming time was(30±1.6),(26±2.1),(27±1.4)d,lespectively,wht that in the MEF group significantly higer than in other two groups(P0.05).The reprogramming efficiencies were 0.3%±0.03%,0.56%±0.08%,0.7% ±0.02% respectively(P0.05).Three kinds of substrate could better support iPSCs growth and make them to maintain un-differentiation status.Conclusion with no heterologous animal components,and the adrantaga of simple pleparation,oonfrollable standard and shorter gramming time is easy to prepare,the standard is controllable and the reprogramming time is shorter,which is an ideal substrate for supporting iPSCs growth.
【Fund】: 国家自然科学基金资助项目(81360161);; 云南省教育厅基金资助项目(2015Y153)
【CateGory Index】: R78
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