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《Chongqing Medicine》 2017-13
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Role of M3 receptor in the effect of penehyclidine hydrochloride up-regulating β-arrestin-1 expression

Cheng Bin;Wang Yipeng;Zhan Jia;Department of Anesthesiology,Zhongnan Hospital of Wuhan University;  
Objective To investigate the role of M3 receptor in the effect of penehyclidine hydrochloride(PHC)upregulatingβ-arrestin-1expression in lipopolysaccharide(LPS)-induced human pulmonary microvascular endothelial cell(HPMVEC)injury.Methods.M3 shRNA transfected HPMVEC and normal HPMVEC cells were randomly divided into LPS group(A),LPS+PHC group(B),LPS+ M3 shRNA transfection group(C)and PHC+LPS+M3shRNA transfection group(D).The cytoskeleton change was observed by laser scanning confocal.The LDH level in cellular supernate was detected.The VCAM-1protein expression was examined by immunofluorescence chemistry.β-arrestin-1protein expression was determined by Western blot andβ-arrestin-1mRNA expression was measured by real-time PCR.Results Compared with the group A or C,F-actin cytoskeleton arrangement in the group B or D was neat,the LDH level and VCAM-1protein expression were decreased,andβ-arrestin-1expression was increased;compared with group A or B,F-actin cytoskeleton arrangement in the group C or D was neat,the LDH level and VCAM-1protein expression were decreased,while theβ-arrestin-1expression had no obvious change.Conclusion Silence M3 receptor is conducive to reduce LPS-induced HPMVEC injury.But the role of PHC up-regulatingβ-arrestin-1expression has no necessary connection with M3 receptor.
【Fund】: 国家自然科学基金青年基金项目(81101408);; 湖北省武汉市科技局晨光计划项目(2016070204010150)
【CateGory Index】: R459.7
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