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《Chongqing Medicine》 2017-13
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Study on effect and mechanism of GSPB2 for protecting vascular endothelial cell and delaying apoptosis

Li Qiang;Yang Chengming;Department of Cardiology,Nanchuan District People′s Hospital;Department of Cardiology,Daping Hospital,Third Military Medical University;  
Objective To observe the protective effect of GSPB2 for delaying the human endothelial cells apoptosis with H_2O_2 induced human endothelial cells apoptosis as the model and to investigate its mechanism.Methods The human endothelial cells were pre-treated by 0.5mmol H_2O_2,then different concentrations of GSPB2(5.0,10.0,20.0μmol/L)was added for protecting cells.The cellular apoptosis was detected by TUNEL method;the apoptosis related molecules,such as Caspase-3,Bax,Bcl-2,and influencing apoptosis related molecules such as PI3 K,p-Akt and Akt protein expression were detected by Western blot;Transwell chamber was used to detect cell migration situation.Results 0.5mmol H_2O_2 induced obvious increase of human endothelial cells(P0.01).GSPB2 could significantly alleviate the H_2O_2 induced apoptosis of human endothelial cells(P0.05).10μmol/mL GSPB2 could be close to return to H_2O_2 untreated control group level.Further found that Caspase-3,Bax protein expression were up-regulaed,Bcl-2expression was down-regulated and the expression of PI3 K,p-Akt and Akt wa sup-regulated.Conclusion The different concentrations of GSPB2 has a protective effect on vascular endothelial cells induced by H_2O_2,moreover which manifested by the concentration and time dependence.Its mechanism is related with the related molecules PI3 K,p-Akt and Akt
【CateGory Index】: R54
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 GUO Bing,QIN Guan-hai,YANG Ying(Chongqing Medical and Pharmaceutical College,Chongqing400030,China);Effects of α-tocopherol on streptozotocin-induced apoptosis of neonatal rat pancreatic cells[J];重庆医学;2010-16
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 Li Qiang;Yang Chengming;Department of Cardiology,Nanchuan District People′s Hospital;Department of Cardiology,Daping Hospital,Third Military Medical University;;Study on effect and mechanism of GSPB2 for protecting vascular endothelial cell and delaying apoptosis[J];重庆医学;2017-13
2 LI Nai;FAN Chen;LI Xiaobo;LONG Limei;CAO Xueli;School of Food and Chemical Engineering /Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients,Beijing Technology and Business University;;Optimization of Determination Method and Analysis on Tocopherol Content in Edible Vegetable Oils[J];食品科学技术学报;2015-03
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