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《Chongqing Medicine》 2017-13
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Study on immunocytochemistry p16/Ki-67 double staining combined with DNA ploidy analysis for analyzing and predicting cervical high grade squamous intraepithelial lesion

Zhang Jinqiu;Zhu Ping;Lu Minhua;Dong Jianfeng;Yin Yongxiang;Zhao Hua;Department of Pathology,Affiliated Wuxi Municipal Maternal and Child Health Care Hospital,Nanjing Medical University;  
Objective To investigate the diagnostic value of DNA ploidy analysis combined with immunocytochemistry p16/ki-67 double staining in cervical high grade squamous intraepithelial neoplasia(HSIL)and cervical squamous cell carcinoma(SCC).Methods A total of 73 cases of cytological tests were randomly collected.Among them,53 cases were small DNA ploidy abnormal cells and 20 cases were DNA ploidy negative.The p16/Ki-67 results were detected by immunocytochemistry double staining.With the pathological results as the golden standard,the diagnostic values of DNA ploidy analysis and DNA ploidy analysis combined with p16/Ki-67 double staining in HSIL + was contrastively analyzed by pathologic results.Results Among 20 samples of DNA ploidy negative,the p16/Ki-67 double staining results all were negative.The positive predictive value of DNA ploidy analysis for HSIL + was 34.62%.The sensitivity of DNA ploidy analysis combined with p16/Ki-67 double staining for HSIL + was 84.62%,and its specificity was 92.31%,the positive predictive value was 78.57% and the negative predictive value was 94.74%,which were significantly higher than those of DNA ploidy analysis(P0.05).Conclusion p16/Ki-67 double staining can significantly improve the prediction value of HSIL.The DNA ploidy analysis combined with p16/Ki-67 double staining is an effective method for predicting HSIL +,which is suitable for the implementation in the areas with lack of medical resources.
【Fund】: 江苏省无锡市卫计委妇幼健康成果技术推广项目(FYTG2016-2);; 江苏省无锡市卫生计生科技成果和适宜技术推广项目(T201645)
【CateGory Index】: R737.33
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 LIN Dan;LUO Xin;JIANG Xuefeng;The First Affiliated Hospital of Jinan University;The Third Affiliated Hospital of Southern Medical University;;Combining DNA Ploidy Analysis and High Risk Type of Human Papillomavirus Detection to Detect Cervical Lesions[J];实用妇产科杂志;2014-07
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【Co-citations】
Chinese Journal Full-text Database 7 Hits
1 Zhang Jinqiu;Zhu Ping;Lu Minhua;Dong Jianfeng;Yin Yongxiang;Zhao Hua;Department of Pathology,Affiliated Wuxi Municipal Maternal and Child Health Care Hospital,Nanjing Medical University;;Study on immunocytochemistry p16/Ki-67 double staining combined with DNA ploidy analysis for analyzing and predicting cervical high grade squamous intraepithelial lesion[J];重庆医学;2017-13
2 WANG Shuangshuang;LI Hui;TANG Futing;WU Xiaobin;Department of Pathology,Affiliated Hospital of Nanjing University of TCM;;Application of DNA quantitative cytology and high-risk HPV-DNA test in screening cervical lesions[J];临床与病理杂志;2017-04
3 YIN Yong-xiang;ZHAO Hua;LIANG Jie;ZHANG Jin-qiu;ZHAO Min;WANG Jia-jun;Department of Pathology,the Affiliated Maternity and Child Health Hospital of Nanjing Medical University;Department of Gynecology,the Affiliated Maternity and Child Health Hospital of Nanjing Medical University;;Study of predictive value for detection of high-grade cervical intraepithelial neoplasia by p16/Ki-67 dual-stained liquid-based cytology[J];临床与实验病理学杂志;2017-03
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