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《Chongqing Medicine》 2017-14
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Effect of E_2 on the expression of estrogen receptor α,β and VEGF and the proliferation of EOMA cells

Song Xiaofeng;Wang Ning;Li Yasha;Jin Xianqing;Wang Yi;Li Xiaoqing;Zhou Dekai;Department of Gastrointestinal and Neonatal Surgery,the Children′s Hospital,Chongqing Medical University/Ministry of Education Key Laboratory of Child Development and Disorders,Chongqing International Science and Technology Cooperation Center for Child Development and Disorders,Key Laboratory of Pediatrics in Chongqing;  
Objective To explore the effect of 17β-estradiol(E2)on proliferation of murine endothelial(EOMA)cells and the expression of estrogen receptor(ERαand ERβ)and vascular endothelial growth factor(VEGF).Methods Different concentrations of E2(1,10,100nmol/L)were used in EOMA cells.At the same time,We set the control group(without E2).MTT assay was used to detect the effect of EOMA on the growth of EOMA cells at different time points.Western blot was used to detect the expression of ERαandβin EOMA cells;and the secretion level of VEGF in supernatant was detected by ELISA.Results The OD value of E2group(10nmol/L)was significantly higher than that of control group at 36h(P0.05).The ODvalues of E2group(10nmol/L and 100nmol/L)were significantly higher than that of control group(P0.05)at each time,and the OD value was the highest at 36h(P0.01).The expression of ERαand ERP in EOMA cells cultured with E2(10nmol/L)and the expression of ERαin EOMA cells cultured with(100nmol/L)were significantly higher than those in the control group(P0.05).At 24,48 h,the expression of VEGF of each E2 group was higher than that of the control group;and at 48 h,the VEGF levels in the E2 group(10nmol/L and 100nmol/L)were significantly higher than E2group(1nmol/L)(P0.05).Conclusion E2 could promote the proliferation of EOMA cells,mainly through the expression of ERα,VEGF to achieve.
【Fund】: 国家临床重点专科建设项目(国卫办医函[2013]544);; 重庆市自然科学基金资助项目(cstc2011jjA10087);; 重庆市卫生局基金资助项目(2011-2-252);; 重庆市教委科学技术研究资助项目(KJ120308)
【CateGory Index】: R726.5
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