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《Chongqing Medicine》 2017-14
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Effect of miR-204 in cell biological characteristics of breast cancer MDA-MB-231 cell

Wu Liucheng;Du Lili;Wang Jing;Yin Hailin;Ma Chao;Jiang Maorong;Shao Yixiang;Xinglin College,Nantong University;Laboratory Animals Centre,Nantong University;  
Objective To study the effect of microRNA-204(miR-204)on the biological characteristics of breast cancer cells.Methods Real-time PCR was used to detect the expression of miR-204 in human breast cancer cell MDA-MB-231 after transfection of miR-204 mimics and inhibitor for 48 h.Flow cytometry was used to analyse the effect of miR-204 on the proliferation and apoptosis of MDA-MB-231 cells.The effect of miR-204 on the migration of MDA-MB-231 cells was detected by Transwell migration assay.Results Real-time PCR analysis showed that miR-204 mimics and inhibitors had significant effect compared with normal control group(P0.01).Flow cytometry analysis showed that compared with normal control group,the number of G1 phase cells of miR-204 mimics group was significantly decreased(P0.01),while the number of G2/M cells of miR-204 mimics group was significantly increased(P0.01).In contrast,the number of G1 phase cells of miR-204 inhibitor group was significantly increased(P0.01),while the number of G2/M cells of miR-204 inhibitor group was significantly decreased(P0.01).miR-204 mimics group significantly promoted apoptosis,while the inhibitor group significantly inhibited apoptosis(P0.01).Transwell migration analysis showed that the number of cells of miR-204 mimics group were significantly reduced,while the number of cells was significantly increased in the inhibitor group(P0.01).Conclusion We find miR-204,which can promote cell apoptosis and inhibit cell proliferation and migration,is a negative factor in the breast cancer cell line MDA-MB-231.
【Fund】: 江苏省高等学校大学生创新创业训练计划项目(201513993009Y)
【CateGory Index】: Q291
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【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 Wu Liucheng;Du Lili;Wang Jing;Yin Hailin;Ma Chao;Jiang Maorong;Shao Yixiang;Xinglin College,Nantong University;Laboratory Animals Centre,Nantong University;;Effect of miR-204 in cell biological characteristics of breast cancer MDA-MB-231 cell[J];重庆医学;2017-14
2 Ou Lichang;Guo Xiaoping;Meng Yuanzhen;Wuzhou Red Cross Hospital;;Effect of admission and commitment therapy on psychological resilience and disease awareness in patients with breast cancer surgery[J];现代临床护理;2017-02
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1 ZHENG Ying,WU Chun-xiao, WU Fan.Shanghai Municipal Center for Disease Control and Prevention,Shanghai 200336,China;Status and trends of breast cancer mortality in Chinese females[J];中华预防医学杂志;2011-02
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