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《Chongqing Medicine》 2017-14
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Correlation analysis of NF-κB signaling pathway activated by IL-18 in CD4~+ T cells and the pathogenesis of PBC

Zhao Erchuan;Li Hongmei;Ren Zhijing;He Yuqing;Wang Mingzhu;Ye Zhenxuan;Zhou Wenjing;Zhang hua;Clinical Laboratory,Guizhou Medical University;Department of Medical Laboratory,Guizhou Provincial People′s Hospital;Department of Medical Laboratory,Guiyang Nursing Vocational College;  
Objective To explore the correlation between NF-κB signaling pathways activated by IL-18 in CD4+T cells and the pathogenesis of PBC.Methods We detected the expression of IL-18 mRNA in PBMCs,IL-18 level in plasma,receptor IL-18 R on surface of CD4+T cell,proliferation rate of CD4+T cell and its NF-κB signaling pathway protein IκBαand NF-κB p65 by qRTPCR,ELISA,flow cytometry,MACS and Western blot on 32 cases of patients with PBC(PBC group)and 32 healthy people(control group)in Guizhou provincial people′s hospital.Results The level of IL-18 in PBC group was significantly higher than that in control group(P0.05).The relative expression of IL-18 mRNA in PBC group was significantly higher than that in control group(P0.05).The percentage of CD4+T cells expressing IL-18Rαin PBC group was higher than that in control group(P0.05).The proliferation rate of CD4+T cells stimulated by IL-18 in PBC group was significantly higher than that in healthy control group(P0.01).The relative expression levels of NF-κB p65 protein were up-regulated in IL-18,and the expression of IκBαprotein in each group was significantly increased,especially in PBC group(P0.01).Conclusion IL-18 can activate NF-κB signal pathway in CD4+T cells and participate in the pathogenesis of primary biliary cirrhosis.
【Fund】: 贵州省科学技术厅项目(黔科合LH字[2014]7008号)
【CateGory Index】: R446.6
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