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《Chongqing Medicine》 2017-14
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An improved method for isolation of human atrial cardiomyocytes

Wang Xiaoyu;Fan Zhongcai;Zhou Wei;Yu Yiyan;Zhou Tao;Li Miaoling;Department of Cardiology,the Affiliated Hospital of Southwest Medical University;Key Laboratory of Medical Electrophysiology,Ministry of Education of China,Institute of Cardiovascular Research,Southwest Medical University;  
Objective To study an improved isolated method of single human atrial myocytes.Methods Enzyme digestion method was used to isolate single myocytes from human atrial and whole-cell patch clamp technique was used to record small conductance calcium activated potassium current.Results This method obtained a large number of atrial myocytes.The total amount of atrial myocytes in SR group was 320±30while AF group was 230±20and the difference was statistically significant(P0.01).In this study,a large number of simple and striated single atrial myocytes were obtained,and a typical small-conductance calcium-activated potassium channel current was recorded on the isolated atrial myocytes.Conclusion The established isolated method is simple,stable and effective.We can acquire a large amount of single atrial myocytes with good quality.
【Fund】: 国家自然科学基金资助项目(81470022);; 四川省教育厅重点项目(16ZA0192);; 泸州市-泸州医学院联合项目(20140975)
【CateGory Index】: R972.4
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 LI Miao-Ling ZENG Xiao-Rong~* YANG Yan LIU Zhi-Fei DING Yin-Yuan ZHOU Wen PEI Jie Department of Myocardial Electrophysiology,Luzhou Medical College,Luzhou 646000,China;An improved method for isolation of single atrial myocyte from human heart[J];生理学报;2007-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Wang Xiaoyu;Fan Zhongcai;Zhou Wei;Yu Yiyan;Zhou Tao;Li Miaoling;Department of Cardiology,the Affiliated Hospital of Southwest Medical University;Key Laboratory of Medical Electrophysiology,Ministry of Education of China,Institute of Cardiovascular Research,Southwest Medical University;;An improved method for isolation of human atrial cardiomyocytes[J];重庆医学;2017-14
2 WANG Hua;LI Tao;ZHANG Li;LI Miao-ling;YANG Yan;ZENG Xiao-rong;Key Laboratory of Medical Electrophysiology of Ministry of Education,Luzhou Medical College;;Effects of intracellular calcium alteration on SK currents in atrial cardiomyocytes from patients with atrial fibrillation[J];中国应用生理学杂志;2014-04
3 ZHANG Li;LI Tao;LI Miao-ling;MAO Liang;TAN Xiao-qiu;YANG Yan;ZENG Xiao-rong;Key Laboratory of Ministry of Education for Medical Electrophysiology,Institute of Cardiovasology,Luzhou Medical College;;Protein kinase A regulates small-conductance calcium-activated potassium type 2 channel in chronic atrial fibrillation patients[J];中国病理生理杂志;2013-08
4 LI Miao-ling1,TANG Han-qing2,WANG Yong2,GUO Jian2,PANG Yu-zhou3,LI Guo-zhang2(1.Institute of Myocardium Electrophysiology of Luzhou Medical,Lu Zhou 646000,China;2.Beijing University of Chinese medicine,Beijing 100029,China;3.Guangxi University ofChinese medicine,Nanning 530001,China);Effects of matrine on action potential and sodium channel in guinea pig papillary muscles[J];中国中医基础医学杂志;2012-11
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6 Li Tao,et al Department of Electrophysiology,Institute of Cardiovasology,Luzhou Medical College;EFFECT OF SODIUM CONCENTRATION ON NA~+ CURRENT IN CULTURED CARDIOMYOCYTES OF SUCKLING MOUSE[J];泸州医学院学报;2011-04
7 LI Miao-ling~*,LI Tao,LEI Ming,TAN Xiao-qiu, YANG Yan,LIU Tai-peng,PEI Jie,ZENG Xiao-rong.* Intitute of Myocardium Electrophysiology of Luzhou Medical College,Luzhou 646000,China;Increased small conductance calcium-activated potassium channel(SK2 channel) current in atrial myocytes of patients with persistent atrial fibrillation[J];中华心血管病杂志;2011-02
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9 Zhao Chaoren,Zhou Huafu,Feng Xun,Li Yi,Qian Jun,Xu Xianzeng.(Department of Cardiothoracic Suegery,the First Hospital Affiliated of Guangxi Medical University,Nanning 530021,China);THE DIVERSIFICATION OF ATRIAL MYOCYTEIN ACTION POTENTIAL IN PATIENTS WITH ATRIAL FIBRILLATION[J];广西医科大学学报;2011-01
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【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 LI Miao-ling, ZENG Xiao-rong, YANG Yan, LIU Zhi-fei,ZHOU Wen,DING Yin-yuan, PEI Jie. Department of Myocardial Electrophysiology, Institute of Cardiovascular Research, Luzhou Medical College, Luzhou 646000, China;Research of L-type Ca~(2+) channel current in atrial myocytes of patients with chronic atrial fibrillation[J];中华心血管病杂志;2006-04
2 ZHANG Biao~1, ZENG Xiao-rong~2, YANG Yan~2, LIU Zhi-fei~2, TONG Lin~3 (~1Department of Physiology, Guangdong Medical College, Zhanjiang 524023, China;~2Institute of Myocardial Electrophysiology, Luzhou Medical College, Luzhou 646000, China;~3The Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China);Acetylcholine sensitive K~+ channel (K_(ACh)) in chronic human atrial fibrillation[J];中国病理生理杂志;2006-04
3 ZHANG Yu, ZENG Xiao-rong, YANG Yan, ZHANG Biao, LIU Zhi-fei, LI Miao-ling, ZHOU Wen, PE1 Jie. Institute of Myocardium Electrophysiology, Luzhou Medical College, Luzhou 646000,Sichuan, China;Human inward rectifying potassium current and Kir2.1 mRNA expression in myocytes isolated from patients with chronic atrial fibrillation[J];中华心血管病杂志;2006-01
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