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《Chongqing Medicine》 2017-14
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Analysis of amplification and bioinformatics on mycobacterium tuberculosis protein higA

Dong Na;Liu Dan;Fu Yurong;Yi Zhengjun;Department of Medical Laboratory,Weifang Medical University;Department of Pathogen Biology,Weifang Medical University;  
Objective To amplify the higA gene from the Mycobacterium tuberculosis,and to analyze the structure and function of their encoded proteins by using bioinformatics.Methods Total DNA was extracted from Mycobacterium tuberculosis.PCR of higA was performed and the products were sequenced.The biological features of the higA protein including,its physical and chemical properties,signal peptide,spatial structure and epitopes were analyzed by using software online.Results The PCR products of higA were 450 bp in length,which were consistent with the expected size.The higA protein consisted of 149 amino acids and had the following characteristics:a theoretical isoelectric point of 7.93,a fat-soluble factor of 94.30,and instability coefficient of36.57.The higA protein had no signal peptide,containing 10 phosphorylation sites and multiple potential epitopes.Conclusion Mycobacterium tuberculosis higA gene can be amplified by PCR and the characteristics of higA protein is identified.
【Fund】: 国家自然科学基金资助项目(81470001 81170080)
【CateGory Index】: R378.91
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 YUAN Fang;LIAO Ze-rong;ZHANG Lu;CHEN Yuan-xiao;Department of Biochemistry and Molecular Biology,Kunming Medical University;Modern Education Technology Center,Kunming Medical University;Department of Cell Biology and Medical Genetics;;Bioinformatic analysis of the structure and function of FadD3 from Mycobacterium tuberculosis[J];中国病原生物学杂志;2015-05
2 ZHAO Ya-jing;BAI Xue-juan;LIANG Yan;ZHANG Jun-xian;YANG You-rong;ZHAO Wei-guo;WU Xue-qiong;Hebei North University;Key Laboratory for Tuberculosis Control,Tuberculosis Research Institute,309th Hospital of the Chinese PLA;;Prediction of epitopes of Mycobacterium tuberculosis Rv3407 protein[J];中国病原生物学杂志;2014-06
【Co-citations】
Chinese Journal Full-text Database 9 Hits
1 Dong Na;Liu Dan;Fu Yurong;Yi Zhengjun;Department of Medical Laboratory,Weifang Medical University;Department of Pathogen Biology,Weifang Medical University;;Analysis of amplification and bioinformatics on mycobacterium tuberculosis protein higA[J];重庆医学;2017-14
2 ZHAN Wei-hong;WU Qi-hang;ZHAO Long-qi;WANG Xin-qian;SUN Yan;YU Xiao-li;School of Biology and Pharmaceutical Engineering,Wuhan Polytecnic University;;Prediction and analysis of T,B cell epitopes's distribution of the Mycobacterium tuberculosis Rv2181[J];武汉轻工大学学报;2017-01
3 BAO Meng;FU Yu-rong;YI Zheng-jun;Department of Medical Laboratory,Weifang Medical University;,School of Clinical Medine,Weifang Medical University;;Expression and bioinformatics analysis of LysinB of mycobacteriophage D29[J];中国病原生物学杂志;2017-02
4 WANG Xin-qian;SUN Yan;ZHAN Wei-hong;LEI Hang;CHEN Gao-zhan;YU Xiao-li;School of Biology and Pharmaceutical Engineering,Wuhan Polytecnic University;;Prediction and analysis of T cell epitopes's distribution of the Mycobacterium tuberculosis Rv3607c[J];武汉轻工大学学报;2016-02
5 LU Xiao-hong;FU Yu-rong;YI Zheng-jun;Department of Laboratory Medicine,Weifang Medical University;Department of Medical Microbiology,Weifang Medical University;;Bioinformatic analysis of MT3876 gene in Mycobacterium tuberculosis[J];中国病原生物学杂志;2016-04
6 Bao Meng;Fu Yurong;Yi Zhengjun;Department of Medical Laboratory,Weifang Medical University;School of Clinical Medicine,Weifang Medical University;;Analysis of serum lipids of patients with pulmonary tuberculosis and bacterial pneumonia[J];重庆医科大学学报;2017-03
7 CHEN Gao-zhan;SUN Yan;ZHAN Wei-hong;LEI Hang;WANG Xin-qian;SONG Yan-zheng;ZHANG Shu-lin;YU Xiao-li;School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University;Shanghai Clinical Center for Public Health;Department of Immunology and Microbiology,School of Medicine,Shanghai Jiao Tong University;;Sequencing of a homologue of mmpL3 from Gordonia bronchialis and prediction of its function[J];中国病原生物学杂志;2016-02
8 WANG Yuanyuan;DONG Xiaolin;SUN Yu;LI Zhiping;JIN Zhao;XIA Zhiping;College of Veterinary Medicine,Jilin University;Military Veterinary Institute,Academy of Military Medical Sciences of PLA;;Baculovirus Expression and Bioinformatic Analysis of Rabies Virus Glycoprotein[J];吉林农业大学学报;2016-02
9 WANG Xu-dong;NIE Heng;WU Li-xian;Department of Microbiology and Immunology of Dali University In Yunnan Province;;A comparative study of PFGE and MLVA to genotype Mycobacterium tuberculosis infecting patients with HIV in Dali District[J];中国病原生物学杂志;2015-12
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 YUAN Fang;CHEN Yuan-xiao;Stephanie Dawes;Edward N.Baker;Department of Biochemistry and Molecular Biology,School of Basic Medicine,Kunming Medical University;Department of Cell Biology and Medical Genetics,School of Basic Medicine,Kunming Medical University;Maurice Wilkins Centre for Molecular Biodiscovery and School of Biological Sciences,University of Auckland;;Cloning and expression of(fatty Acyl-CoA synthetase) gene FadD3 in Mycobacterium tuberculosis H37Ra[J];云南大学学报(自然科学版);2014-04
2 WANG Guang-xiang,SHANG You-jun,LV Zhan-lu,ZHANG Ke-shan,TIAN Hong,LIU Xiang-tao (Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences / State Key Laboratory of Veterinary Etiological Biology / National Foot-and-Mouth Disease Reference Laboratory / Key Laboratory of Animal Virology,Ministry of Agriculture of China,Lanzhou 730046,China);Prediction of secondary structures and cell epitopes of F1L protein of ORFV[J];中国人兽共患病学报;2012-12
3 SONG Shuai,LI Chun-ling,JIA Ai-qing,YANG Dong-xia,LI Miao(Guangdong Open Laboratory of Veterinary Public Health,Institute of Veterinary Medicine,Guangdong Academy of Agricultural Science,Guangzhou,Guangdong,510640,China);Progress on Approaches for Antigen Epitope Study[J];动物医学进展;2010-12
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