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《Chongqing Medicine》 2017-15
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Influence of blocking gastrin receptor on the proliferation and apoptosis and expression of key proteins in related pathway of gastric cancer cell

Yuan Hang;Wang Chuang;Wang Qinrong;Zhao Yan;Li Yajie;Long Niya;Zhou Jianjiang;Key Laboratory of Molecular Biology of Guizhou Medical University/Key Laboratory of Endemic and Minority Diseases,Ministry of Education Guiyang;  
Objective To investigate the effects of blocking gastrin receptor on the proliferation,apoptosis and expression of key proteins in the related pathway in gastric cancer cell lines.Methods In the experimental group,the gastric cancer cell lines SGC-7901 and AGS cells were treated with 5mmol/L proglumide,a kind of a gastrin receptor antagonist.And the normal cultured gastric cancer cells SGC-7901 and AGS were used in control group.The growth of each group was detected by MTT assay;the cell growth curve was drawn by flow cytometry;the cell cycle of each group was detected by flow cytometry.Annexin V-FITC/PI double staining was used to detect the cell growth of apoptosis.The relative mRNA expression ofβ-catenin,nuclear factor-P65,mammalian target of rapamycin and glycogen synthase kinase 3beta in Wnt,NF-κB and PI3K-AKT-MTOR pathways were detected by RTqPCR.The expression ofβ-catenin protein was detected by Western blotting.Results After treatment with proglumide,the growth of the cells in the experimental group was lower than that in the control group;and the proportion of S phase cells in the cell cycle was also lower than that in the control group,but the proportion of cells in G_0/G_1 phase was higher than that in the control group(P0.05).The percentage of apoptotic cells was also increased after treatment with proglumide(P0.05).Furthermore,proglumide treatment significantly reduced the expression ofβ-catenin at both mRNA and protein levels(P0.05).Conclusion Blocking gastrin receptor can down-regulate the expression ofβ-catenin,inhibit the cell proliferation and promote the cell apoptosis in gastric cancer cells.
【Fund】: 国家自然科学基金资助项目(81260303 31560326);; 贵州省科技合作计划项目(黔科合LH字[2015]7360)
【CateGory Index】: R735.2
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