Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chongqing Medicine》 2017-15
Add to Favorite Get Latest Update

The effect of USP4 on the proliferation of human hypertrophic scar fibroblasts

Chen Yan;Zhang Jie;Department of Skin Surgery,Sir Run Run Shaw Hospital School of Medicine,Zhejiang University;Oral and Maxillofacial Plastic Surgery,the First Affiliated Hospital of Nanchang University;  
Objective To investigate the effects of USP4 on the proliferation of human hypertrophic scar fibroblasts(HSFB).Methods HSFB were cultured in vitro.The fourth generation of HSFB in logarithmic growth phase was selected in the experiment.HSFB were intervened by Vialinin A which was the inhibitor of USP4 for 0,12,24,48 h,then collected cells and the their expression of USP4 and TβRI and Smad7 protein was detected by Western blot.MTT assay was used to detect the effect of Vialinin A on the proliferation of HS fibroblasts and the cells were divided into experimental group and control group(without intervention).Results After treatment with Vialinin A in HSFB,the expression of USP4 and TβRI protein in HSFB decreased gradually,especially in 12h(P0.05),and Smad7 protein expression was increased gradually(P0.05).The proliferative activity of intervened HSFB reduced gradually,the difference between experimental group and control group was statistically significant(P0.05).Conclusion USP4 might inhibit the proliferation of scar cells and down-regulation of USP4 expression in hyperplastic scar fibroblasts,which can slow proliferative activity of intervened HSFB by regulating TGF-β/Smad signaling pathway.
【Fund】: 国家自然科学基金资助项目(81460295);; 江西省卫生计生委科技计划资助项目(20155201)
【CateGory Index】: R622
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Yanchang Li;Yuan Gao;Zhongwei Xu;Qiuyan Lan;Ping Xu;Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, National Center for Protein Sciences Beijing, Beijing Proteome Research Center, National Engineering Research Center for Protein Drugs;Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences;;Application of proteomics in deubiquitinases research[J];生物工程学报;2014-09
2 LIN Zun-wen,XU Shao-hong,YOU Min,LIU De-wu,ZHONG Shi-zhen.Department of Human Anatomy,Southern Medical University,Guangzhou 510515,China Corresponding author: LIU De-wu E-mail: dewuliu@126.com;Refined culture and identification of human hypertrophic scar fibroblasts[J];实用医学杂志;2011-24
【Co-citations】
Chinese Journal Full-text Database 3 Hits
1 Chen Yan;Zhang Jie;Department of Skin Surgery,Sir Run Run Shaw Hospital School of Medicine,Zhejiang University;Oral and Maxillofacial Plastic Surgery,the First Affiliated Hospital of Nanchang University;;The effect of USP4 on the proliferation of human hypertrophic scar fibroblasts[J];重庆医学;2017-15
2 Yang Lutao;Liu Meiling;Zhang Youlai;Xin Guohua;Li Guohui;Zeng Yuanlin;Burn Center,the First Affiliated Hospital,Nanchang University;;Effect of sodium-selenite on human hypertrophic scar fibroblasts proliferation in vitro[J];重庆医学;2014-35
3 LIAN Min;YE Yanqing;WANG Tian;LIU Bing;Department of Respiratory Medicine,Zhongnan Hospital of Wuhan University;;Improved Separation Method and Identification of Primary Fibroblasts from New Born Sprague Dawley Rats[J];氨基酸和生物资源;2014-01
【Secondary Citations】
Chinese Journal Full-text Database 7 Hits
1 GAO Xue1,ZHANG XueLi1,ZHENG JunJie1 & HE FuChu1,2 1 State Key Laboratory of Proteomics,Beijing Proteome Research Center,Beijing Institute of Radiation Medicine,Beijing 102206,China;2 Institutes of Biomedical Sciences,Fudan University,Shanghai 200032,China;Proteomics in China:Ready for prime time[J];Science China(Life Sciences);2010-01
2 Jin-ming YANG Department of Pharmacology,The Cancer Institute of New Jersey,University of Medicine and Dentistry of New Jersey/Robert Wood Johnson Medical School,New Brunswick,New Jersey 08903,USA;Emerging roles of deubiquitinating enzymes in human cancer[J];Acta Pharmacologica Sinica;2007-09
3 Liu Dewu, Li Guohui, Zou Ping 1, Liu Deming 2 (Burn Center, 1Department of Pharmacy, The First Affiliated Hospital of Jiangxi Medical College, Nanchang 330006; 2Department of Anatomy, Jiangxi Medical College,Nanchang 330006);Inhibitory effect of tetrandrine on the proliferation and collagen synthesis of human scar-derived fibroblasts stimulated by PDGF and TGF-β[J];中药药理与临床;2004-04
4 DAI Zhen-sheng 1,CHEN Bao-an 2, XU Yan-li 1, HUANG Pei-lin 3, XIE Yi 4,XIE Hong 5 (1.Department of Hematology,Affiliated Nanjing First Hospital,Nanjing Medical University,Nanjing 210006,China; 2.Department of Hematology,Affiliated Zhongda Hospital,Southeast University,Nanjing 210009,China; 3.Department of Pathology and Pathophysiology,School of Basic Medical Science,Southeast University,Nanjing 210009,China; 4.Department of Hematology,Affiliated Huashan Hospital,Fudan University,Shanghai 200040,China; 5.Institution of Cellbiology,Chinese Academy of Science,Shanghai 200040,China);Modification of the traditional method of fibroblast primary culture[J];东南大学学报(医学版);2004-04
5 ZHANG Jing-De, XING Xin, SUN Mei-qing, SONG Jian-xing,OUYANG Tian-xiang.Department of Plastic Surgery, Changhai Hospital, the Second Military Medical University Shanghai 200433, China;The effects of chitosan on different-derived fibroblasts[J];中华整形外科杂志;2004-01
6 LIU Dewu, LI Guohui, LIU Dealing, CAO Yong, Department of Burns, The First Affiliated Hospital, Jiang Xi Medical College, Nan- chang 330006,Jiang Xi Province,P. R. China.;Effects of tetrandrine on the synthesis of collagen and scar-derived fibroblast DNA[J];中华烧伤杂志;2001-04
7 Liu Dewu,Li Guohui,Cao Yong,Su Ziyi,Wang Wen and Wu Xieqing (Burn Institute,The First Affiliated Hospital of Jiangxi Medical College,Nanchang 330006);Observation on Inhibitory Effect of Tetrandrine on Growth of Human Skin Fibroblast in Vitro[J];中国中药杂志;1998-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved