Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chongqing Medicine》 2017-15
Add to Favorite Get Latest Update

The influence of thyroid hormones on the expression of NGF and BDNF after cerebral ischemia-reperfusion injury in rats

Dou Chao;Zhang Min;Zhao Yuanzheng;Guo Yapei;Wu Shitao;Liu Hengfang;Department of Neurology,the Fifth Affiliated Hospital of Zhengzhou University;  
Objective To investigate the neuroprotective effect of thyroid hormones T3 on cerebral ischemia-reperfusion injury in rats and its mechanism.Methods SD rats were divided into four groups:sham+saline group,sham+T3group,MCAO+saline group,MCAO+T3group.The cerebral ischemia-reperfusion injury rat models were established by right middle cerebral artery occlusion.Thyroid hormones(10μg/100g)or normal saline were given respectively by intraperitoneal injection twice at 1hafter the onset of ischemia and 6hafter reperfusion.Neurobehavioral score was evaluated at 24 hafter reperfusion;TTC staining was used to label infarction area;RT-PCR was used to detect the mRNA level of nerve growth factor(NGF)and brain derived neurotrophic factor(BDNF)in brain tissue;Western blot was employed to determine alterations in protein levels of NGF and BDNF.Results Compared with MCAO+saline group,the neurological deficit and the volume of cerebral infarction of MCAO+T3group was decreased,and the mRNA and protein expression of NGF and BDNF of MCAO+T3group were increased(P0.05).ConclusionThyroid Hormones could promote the nerve repair,stimulate the nerve regeneration and improve the nervous behavioral function by up-regulating the expression of NGF and BDNF.
【Fund】: 河南省医学科技攻关计划项目(16A310021)
【CateGory Index】: R743
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved