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《Chongqing Medicine》 2017-15
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Effect of ghrelin on proliferation and mitofusin-2 expression of human aortic smooth muscle cells

He Yongming;Song Mingbao;Hu Jianbo;Zhang Yuanping;Li Youmei;Department of Cardiology,Xinqiao Hospital of Third Medical Military University;  
Objective To investigate the effects of ghrelin on proliferation of vascular smooth muscle cells(VSMC)and the expression of mitochondrial fusion 2(Mfn-2)in cultured human aortic smooth muscle cells(HASMCs).Methods HASMCs were cultured in vitro,treated with different concentrations(10~(-9),10~(-8),10~(-7),10~(-6),10~(-5) mol/L)ghrelin or 10~(-6) mol/L ghrelin for different time(0,6,12,18,24h).Subconfluent HASMCs at passage 4-6were used in experiments.MTT essay was used to investigate the effect on proliferation of HASMCs.RT-PCR and Western blot were used to analyse the expression of Mfn-2.Results 10~(-7)-10~(-5) mol/L ghrelin inhibited the proliferation of HASMCs,and the inhibitory effect of concentration of 10~(-6) mol/L was the most obvious(P0.01).Ghrelin inhibited the proliferation of HASMCs in 6-24 h,and it reached the peak at 24h(P0.01).10~(-6)mol/L ghrelin significantly increased the expression of Mfn-2mRNA and protein(P0.01).The up-regulation of 10~(-6) mol/L ghrelin on Mfn-2mRNA and protein expression reached the peak at 18h(P0.01).Conclusion Ghrelin might inhibit the proliferation of HASMC by up-regulating the expression of Mfn-2.
【CateGory Index】: R54
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1 He Yongming;Song Mingbao;Hu Jianbo;Zhang Yuanping;Li Youmei;Department of Cardiology,Xinqiao Hospital of Third Medical Military University;;Effect of ghrelin on proliferation and mitofusin-2 expression of human aortic smooth muscle cells[J];重庆医学;2017-15
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