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《Chongqing Medicine》 2017-16
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Research on influence of TLR9 on pancreatic cancer nude mouse model growth and resistance to chemotherapy

Liu Yu;Lei Zehua;Du Bo;Wang Zhixu;Gao Fengwei;Wang Qing;Zhang Jianxin;Department of General Surgery,Leshan Municipal People′s Hospital;Affiliated Hospital of Jiangsu University;  
Objective To observe the effects of TLR9 on the nude mouse transplanted tumor growth of human pancreatic cancer and its drug resistance.Methods The nude mouse transplated tumor of human pancreatic cancer PANC-1was established and randomly divided into 6groups for conducting the experiment:sterile normal saline group,TLR9 agonist,TLR9inhibitor group,gemcitabine group,TLR9 inhibitor plus gemcitabine Bin group,TLR9 agonist plus gemcitabine.The tumor size and growth situation were recorded by the vernier caliper.The immunohistochemical method was used to detect tumor TLR9 receptor expression.The tumor growth,metastasis and paracancerous tissue invasion situation were observed by the magnetic resonance imaging(MRI).Results The volume and growth speed of resected tumor mass in the gemcitabine group,TLR9agonist+ gemcitabine group,TLR9 inhibitor plus gemcitabine group was significantly smaller than those in other groups(P0.05),which in the TLR9agonist+ gemcitabine group were significantly greater than those in the TLR9 inhibitor plus gemcitabine group and gemcitabine group(P0.05),the difference between the TLR9 inhibitor plus gemcitabine group and gemcitabine group had statistical significance(P0.05),while the difference among the TLR9 agonist group,TLR9 inhibitor group and normal saline group had no stastistical significance(P0.05).The tumor in mice at 7weeks after planting showed oval shape with clear boundary by MRI observation,no obvious metastais and paracancerous invasion were seen in paracancerous tissues no statistically significant,5 weeks,6weeks after planting,seven weeks mice observed in MRI,the tumor into an,state clearly that the transfer of the surrounding tissue,no significant vascular invasion,heart,liver,kidney disease.The TLR9 expression on the surface of tumor tissue was detected and identified.Conclusion Pancreatic cancer nude mouse transplated tumor has definitely positive expression of TLR9,TLR9 activation can significantly decrease the sensitivity of pancreatic cancer to chemotherapy,increases the drug resistance of tumor,on contrary promotes the tumor growth.
【Fund】: 国家自然科学基金资助项目(81070287)
【CateGory Index】: R735.9
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