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《Chongqing Medicine》 2017-16
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Benzopyrene activates ERK1/2 signal pathway to promote extracellular matrix protein deposition of airway smooth muscle cells on airway remodeling

Yuan Yalu;Cheng Yuanxiong;Cao Jing;Lai Wenyan;Cai Kaican;Department of Respiration,Nanfang Hospital,Southern Medical University;Department of Cardiology,Nanfang Hospital,Southern Medical University;Department of Cardiothoracic Surgery,Nanfang Hospital,Southern Medical University;  
Objective To investigate the influence of benzopyrene on extracellular matrix(ECM)protein deposition of human airway smooth muscle cells(HASMC)and the related pathway mechanism.Methods HASMC were primarily cultured and the 2-6generations cells were applied in this experiment.The expression amount of ECM gene and protein was detected by real time PCR and Western Blot the phosphorylation level was analyzed by using the Western Blot method.Results Benzopyrene could to increase the expression of HASMC collagenⅠα1protein(P0.01)and ECM protein(including collagenⅠα1,versican,fibronectin,lamininα2)mRNA(P0.05).Benzopyrene could induce the rapid increase of ERK1/2phosphorylation level(P0.01).Furthermore,the ERK pathway inhibitor PD98059 could significantly inhibit the increase of benzopyrene-induced collagenⅠα1(P0.01)and ECM protein(including collagen Ⅰα1,versican,fibronectin,lamininα2)mRNA expression(P0.01).Conclusion Benzopyrene induces the ECM protein deposition of HASMC by activating the ERK1/2pathway,blocking the ERK1/2signal pathway can inhibit the benzopyrene-induced airway remodeling.
【Fund】: 广东省自然科学基金资助项目(s2012010009036);; 南方医院院长基金资助项目(2014A001)
【CateGory Index】: R563.9
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