Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chongqing Medicine》 2017-17
Add to Favorite Get Latest Update

Effects of myocardial PPAR-gamma expression change on reperfusion arrhythmias in ischemia/reperfusion rats

Chen Hua;Mo Xinling;He Xueshu;Xie Ting;The Second Affiliated Hospital of Guilin Medical College;Affiliated Hospital of Guilin Medical College;  
Objective To investigate the effects of PPAR-gamma expression on reperfusion arrhythmias in different preconditioning myocardial ischemia/reperfusion(I/R)animal model.Methods Thirty-two SD rats were randomly divided into 4groups(n=8):rosiglitazone+I/R group(ROS group),GW9662+I/R group(GW group),I/R group and sham group(Sham group).The I/R animal model was constructed by ligation of the left anterior descending coronary artery,with ischemia for 30 min and reperfusion for 120 min.The dynamic limb II lead electrocardiogram monitoring was performed;PPAR-gamma mRNA was detected by fluorescent quantitative PCR and the change of PPAR-gamma protein expression was detected by Western blot.Results The increasing range of QRS wave width detected before operation,at 30 min of ischemia,at 1,2hof reperfusion from large to small were in turn the ROS group,I/R group,GW group and Sham group;the reperfusion arrhythmia score in the ROS group was significantly higher than that in the other groups(P0.05),while the GW group was relatively reduced.The expression level of PPAR-gamma mRNA in the ROS group detected by fluorescent quantitative PCR was significantly up-regulated(P0.05),while which in the GW group was down-regulated compared with the I/R group and ROS group(P0.05).The expression of PPAR-gamma protein was similar to that of PPAR-gamma mRNA.Conclusion Up-regulation of myocardial PPAR-gamma expression may increase the occurrence of reperfusion arrhythmias in myocardial I/R animal model.
【Fund】: 广西壮族自治区卫生厅自筹课题(Z2013492)
【CateGory Index】: R542.22
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved