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《Chongqing Medicine》 2017-17
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Experiment research of cinobufacini on TGF-β1-induced epithelial-to-mesenchymal transition in human colorectal carcinoma cells

Shen Guoqiang;Lin Weidong;Chen Xiangfang;Department of Traumatic Surgery,Shanghai Fire Corps Hospital of Chinese People′s Armed Police;Department of Endocrinology,Affiliated Hospital,Second Military Medical University;  
Objective To investigate the effects of cinobufacini on TGF-β1-induced epithelial-to-mesenchymal transition of human colorectal carcinoma cells in vitro.Methods The cultured colorectal carcinoma cell line(SW480)was divided into the control group,TGF-β1(10ng/mL)individual treatment group and co-treatment groups with TGF-β1(10ng/mL)+cinobufacini(2.5,5,10,20,40,80mg/mL),which were cultured in vitro for 48 h.The proliferation of the cells were measured by CCK8 assay.The morphological changes were observed by inverted phase contrast microscope.The ability of cell invasion and migration was detected by Transwell assay.The mRNA and protein expressions of E-cadherin and Vimentin were detected with QRT-PCR and Western Blot.Results(1)Compared with the TGF-β1(10ng/mL)individual treatment group,TGF-β1(10ng/mL)+ cinobufacini(10,20,40,80mg/mL)co-treatment groups significantly had significantly proliferation inhibitory effect on SW480(P0.05).(2)Compared with the normal control group,TGF-β1individual treatment group and TGF-β1(10ng/mL)+ cinobufacini(2.5 mg/mL)group exhibited classical mesenchymal phenotype,while the TGF-β1(10ng/mL)+ cinobufacini(5mg/mL)co-treatment group showed classical epithelial phenotype.(3)The ability of invasion and migration in the TGF-β1(10ng/mL)+ cinobufacini(2.5,5mg/mL)co-treatment group were significantly weakened compared with the TGF-β1individual treatment group(P0.01).(4)QRT-PCR and Western Blot results indicated that compared with the normal control group,the Vimentin expression in the in the TGF-β1individual treatment group was significantly increased and the E-cadherin expression was significantly decreased.Furthermore,compared with the TGF-β1individual treatment group and control group,the Vimentin expression level in the TGF-β1(10ng/mL)+cinobufacini(2.5,5mg/mL)groups was significantly decreased and E-cadherin expression was significantly increased.ConclusionCinobufacini can inhibit TGF-β1-induced cell proliferation in human colorectal carcinoma SW480 cells,and its mechanism may be related with promoting E-cadherin expression increase,meanwhile decreasing the vimentin expression,thus inhibiting the EMT process induced by TGF-β1.
【Fund】: 国家自然科学基金资助项目(81100585)
【CateGory Index】: R735.35
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