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《Chongqing Medicine》 2017-17
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Analysis on results of HCV screening among volunteer blood donors in Guiyang area

Chen Wenxia;Zuo Li;Zhong Jiang;Shen Junfeng;Guizhou Provincial Blood Center;Teaching and Researching Section of Immunology,Basic College,Guizhou Medical University;  
Objective To analyze and compare the anti-HCV reactivity,HCV nucleic acid detection results and HCV recombinantion immunoblot assay(RIBA)confirmatory test results in blood donors.Methods The blood samples collected from the volunteer blood donors from October 2013 to March 2015 were performed the HCV screening by using the domestic ELISA reagents from two different manufacturers and an imported nucleic acid detection reagent and matching detection system.The samples of anti-HCV reactivity or/and NAT detection positive were performed RIBA.Then the results of reactivity detected by two kinds of ELISA reagents,nucleic acid detection reagent and RIBA confirmatory test results were analyzed and compared.Results A total of133959 samples of volunteer blood donors were detected,in which 113 380 samples covered the nucleic acid detection results,the reactivity samples proportion of anti-HCV detection was 0.19%(252/133959),27 cases were positive in NAT detection with the positive detection ratio of 0.02%(27/113 380);the proportion of HCV reactive samples confirmed by RIBA was 19.8%(50/252),the negative proportion was 54.8%(138/252),and the uncertain proportion was 25.4%(64/252);27samples of nucleic acid detection positive were double reagent reactivity in ELISA detection and positive in confirmatory test.The difference among the results of two ELISA reagents,RIBA confirmatory test results and nucleic acid detection results had statistical significance(P0.05).Conclusion The detection strategy selecting twice ELISA+1kind of nucleic acid detection is more secure.Aiming at higher proportion of false positive samples,the follow up system of blood donors should be established for maximizing the retention of blood donors.
【Fund】: 贵州省卫生和计划生育委员会科学技术基金项目(GZWKJ2012-1-093)
【CateGory Index】: R446.6;R512.63
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