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《Chongqing Medicine》 2017-18
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Effect of TRPC6 after astrocytes activation in traumatic brain injury

Zhang Lijun;Feng Hua;Department of Neurosurgery,Chongqing Municipal Emergency Medical Center;Department of Neurosurgery,Southwest Hospital,Third Military Medical University;  
Objective To explore the role of astrocytes transient receptor potential channel 6(TRPC6)in rat brain injury(TBI).Methods Thirty-nine male Sprague-Dawley(SD)rats were divided into the sham operation group,injury group and deferoxamine(DFX)group(n=13).According to the previous model construction scheme established by our research group Feeney method,the rat brain impact injury model was established.The Morris water maze test was performed and the defected brain volume was measured.The immunofluorescence was adopted to detect the co-expression of TRPC6 and GFAP.Then Western blot was performed.Results The defected brain volume after TBI in the DFX group was significantly decreased compared with the injury group[(115.35±13.70)mm3 vs.(209.99±16.70)mm3]](P0.05).The Morris water maze test found that the platform search strategy and search time in the DFX group were(3.13±0.35)and(36.15±26.63)s,which were significantly improved compared with(2.13±0.64)and(110±47.34)s in the injury group(P0.05).The immunofluorescence found that GFAP in the DFX group was highly expressed,moreover the co-expression with TRPC6 was increased.Western blot found that TRPC6 in the DFX group was significantly down-regulated(P0.01).Conclusion In rat TBI early stage,strocytes are activated after DFX treatment and TRPC6 is highly expressed,playing a neuroprotective role.
【Fund】: 国家自然科学基金青年基金项目(30901545);; 重庆市自然科学基金项目(cstc2015jcyjA1051);; 重庆市卫计委临床重点专科项目(渝卫医函2015-517)
【CateGory Index】: R651.15
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 Kim Mai Lee;Andrew G Mac Lean;Department of Microbiology and Immunology,Tulane University School of Medicine;;New advances on glial activation in health and disease[J];World Journal of Virology;2015-02
2 Zhang Lijun,Hu Rong,Li Fei,Meng Hui,Lin Jiangkai,Zhu Gang,Wang Xianrong,Feng Hua(Department of Neurosurgery,Institute of Neurosurgery,Key Laboratory of Neurotrauma Prevention and Treatment,Southwest Hospital,Third Military Medical University,Chongqing,400038,China);Deferoxamine attenuates traumatic brain injury in rats[J];第三军医大学学报;2012-23
3 Zhang Lijun,Hu Rong,Li Fei,Meng Hui,Lin Jiangkai,Zhu Gang,Wang Xianrong,Feng Hua(Department of Neurosurgery,Institute of Neurosurgery,Key Laboratory of Neurotrauma Prevention and Treatment,Southwest Hospital,Third Military Medical University,Chongqing,400038,China);Role of transient receptor potential channels 6 in iron metabolism in rats after traumatic brain injury[J];第三军医大学学报;2012-23
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Zhang Lijun;Feng Hua;Department of Neurosurgery,Chongqing Municipal Emergency Medical Center;Department of Neurosurgery,Southwest Hospital,Third Military Medical University;;Effect of TRPC6 after astrocytes activation in traumatic brain injury[J];重庆医学;2017-18
2 MIN Fang-yuan;LI Xin;WANG Shan;Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Central South University;;Astrocyte dysfunction and central nervous system diseases[J];中南药学;2017-04
3 QIAN Hong;LIU Li-jing;XIE Ming;WU Heng;LIU Shuang-xi;WU Bin;XIANG Hai;School of Clinical Medicine,Hunan University of Medicine;Dept.of Neurology,the First Affiliated Hospital,University of South China;Dept.of Neurology,Huaihua First People's Hospital;;siRNA silencing EGFR expression alleviates brain injury stems from intracerebral hemorrhage of rats[J];基础医学与临床;2016-12
4 DAI Jin-quan;CHEN Yan-hui;Department of Pediatrics,Fuzhou Children's Hospital of Fujian Province;Department of Pediatrics,Fujian Medical University Union Hospital;;The Study of Specific Biological Markers on Brain Injury of Epilepsy[J];医学综述;2016-21
5 GUO Min-fang;ZHAGN Hui-yu;LIU Hua;Department of histology and embryology, Medical School, Shanxi Datong University;;In Astrocyte Stimulated with Lipopolysaccharide[J];中外医疗;2016-17
6 DONG Li-sha;LI Xue-hui;LIU Wen-li;ZHAO Peng;YAO Bi-yun;ZHOU Zong-can;School of Public Health,Peking University;;The activation of primary astrocytes induced by manganese chloride[J];毒理学杂志;2016-01
7 LUO Xiao-jin;LIN Mao;WANG Chun-mei;Department of Physiology,Zhuhai Campus of Zunyi Medical University;;Role and research progress of inflammation in the mechanism of Alzheimer′s disease[J];热带医学杂志;2016-01
8 QIAN Hong;LIU Li-jing;LI Yan-chun;XIE Ming;WU Heng;LIU Shuang-xi;WU Bin;School of Clinical Medicine,Hunan University of Medicine;Dept of Neurology,the First Affiliated Hospital,Hunan University of Medicine;Dept of Neurology,the First Affiliated Hospital,University of South China;Dept of Neurology,Huaihua First People's Hospital;;EGFR siRNA inhibits activation of astrocytes derived from rats through blockade of STAT3 phosphorylation[J];中国药理学通报;2016-02
9 Zhang Lijun;Xiao Hong;Zhou Wei;Liu Ke;Department of Neurosurgery ,Chongqing Municipal Emergency Medical Centre;;Effect observation of giving deferoxamine at different time on experimental craniocerebral traumatic injury in rats[J];现代医药卫生;2016-01
10 DENG Zhi-jing;GUO Zhe;PANG Yi-min;Guangxi Medical University Pharmaceutical School;;Activating rules of astrocytes and microglias in mice after cerebral ischemia[J];中华老年心脑血管病杂志;2016-01
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 Zhang Lijun,Hu Rong,Li Fei,Meng Hui,Lin Jiangkai,Zhu Gang,Wang Xianrong,Feng Hua(Department of Neurosurgery,Institute of Neurosurgery,Key Laboratory of Neurotrauma Prevention and Treatment,Southwest Hospital,Third Military Medical University,Chongqing,400038,China);Deferoxamine attenuates traumatic brain injury in rats[J];第三军医大学学报;2012-23
2 Tong Liu1, Yong-Jing Gao1,2, Ru-Rong Ji1 1 Sensory Plasticity Laboratory, Pain Research Center, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA 2 Institute of Nautical Medicine, Nantong University, Nantong 226001, China;Emerging role of Toll-like receptors in the control of pain and itch[J];Neuroscience Bulletin;2012-02
3 GONG Guo-qi,MENG Hui,FENG Hua (Department of Neurosurgery,Southwest Hospital,Third Military Medicine University,Chongqing 400038,China);Role of iron ion in development of hydrocephalus in rats[J];第三军医大学学报;2008-23
4 GONG Guo-qi,MENG Hui,XIA Yong-zhi,FENG Hua(Department of Neurosurgery,Southwest Hospital,Third Military Medicine University,Chongqing 400038,China);Experimental chronic hydrocephalus after intraventricular hemorrhage in rats:establishment of animal model and pathological observation[J];第三军医大学学报;2008-10
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