Effect of gene transfecting at different time on expression of cyclins in mandibular distraction area
Yin Kang;Hu Chunbing;Zhou Bing;Wu Guoping;Zhao Liping;Department of Surgery,Affiliated Hospital of Southwest Medical University;Department of Burn and Plastic Surgery,Affiliated Hospital of Jining Medical University;Affiliated Friendship Plastic Surgery Hospital,Nanjing Medical University;
Objective To investigate the optimal time and molecular mechanism of gene therapy for promoting distraction osteogenesis by observing the effect of gene transfecting at different time on expression of cyclins in mandibular distraction area.Methods Forty eight New-Zealand rabbits were employed.After accomplishing bilateral mandibular distraction osteogenesis model,the rabbits were randomly divided into the group A,B,C and D.The group A,B and C were transfected by recombinant plasmids pIRES-hBMP2-hVEGF165 2μg(0.1μg/μL)at the distraction area instantly after operation,on postoperative 4,14 dand given local electroporation stimulation.The four groups entered the consolidation stage after 10 dcontinuous traction at a rate of 1mm/d on postoperative 4d.Three rabbits in each group were respectively sacrificed on 7,14,28,56 dof the consolidation stage.The expression of cyclin A,D1,and E of fresh bone tissue in distraction area were detected by immunohistochemical staining.Results Cyclin A,D1,and E were strongly expressed in the traction gap on 7,14 dof consolidation stage found,which was strongest on 7d,moreover the group A,B and C were stronger than the group D.The expression in each group was weakened on 28,56 d.The expression on 7din the group B was stronger than that in the4 group A,C and D(P0.05),the expression had no statistical difference between the group A and C(P0.05),but all were stronger than the group D(P0.05);the expression in the group B and C on 14 d was stronger than that in the group A and D,but the expression had no statistical difference between the group B and C and between the group A and D(P0.05).Conclusion The high expression of cyclin A,D1 and E can promote the cellular division,proliferation and differentiation in distraction area,thus accelerates the new bone formation in the distraction area,prompting that the distraction period is the best time for distraction osteogenesis under gene therapeutic intervention.
【Fund】： 国家自然科学基金资助项目(30600653);; 四川省卫生厅科研课题(12226)
【CateGory Index】： R782
【CateGory Index】： R782