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《Chongqing Medicine》 2017-20
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Protective effect of epigallocatechin-3-gallate on intestine ischemia reperfusion injury

Yang Xukun;Shen Qing;Du Yu;Department of ICU,West China Fourth Hospital,Sichuan University;Department of Radiology,363 Hospital of China Aviation Industry;  
Objective To investigate the effect and mechanism of epigallocatechin-3-gallate(EGCG)on intestine ischemia reperfusion injury(IRI)in rats.Methods Forty SD rats were randomly and equally divided into 4groups:sham group(Sham),intestinal ischemia reperfusion injury group(IRI),EGCG pretreatment group(EGCG)and HLY78group(Wnt-Ag).The IRI,EGCG and WNT-AG groups were performed the superior mesenteric artery(SMA)ligation for 45 min by non-injury vascular clamp to construct the IRI model.EGCG(50 mg/kg)was administrated by intraperitoneal injection at 45 min before ischemia in EGCG group.The Wnt-Ag group was administrated by intraperitoneal injection of EGCG(50mg/kg)plus Wnt-Ag(5mg/kg)at 45 min before ischemia.The IRI group and Sham group were administrated by same dosage of normal saline.The pathological morphology of intestinal tissue was observed by staining at 4hafter reperfusion.The cellular apoptosis was detected by immunohistochemistry.The expressions of tumor necrosis factor-α(TNF-α),interleukin-1(IL-1),interleukin-6(IL-6)in the serum and intestinal tract were examined by ELISA and RT-PCR.The expressions of Wnt,β-catenin,p53,Bax and BCL-2were measured by Western blot.Results Compared with the Sham group,the expression of IL-6,IL-1,TNF-α,Wnt,β-catenin,Bax,cell apoptosis and pathological change of intestinal tract in the IRI group were significantly increased,while the expression of BCL-2was significantly decreased.Compared with the IRI group,the expression of IL-6,IL-1,TNF-α,Wnt,β-catenin,p53,Bax,cell apoptosis and the pathological change of intestinal tracrt in the EGCG group were significantly decreased,while the expression of BCL-2 was significantly increased.Compared with the EGCG group,the expression of IL-6,IL-1,TNF-α,Wnt,β-catenin,Bax,cell apoptosis and pathological change of intestinal tract in the Wnt-Ag group were increased,while the expression of BCL-2was significantly decreased.Conclusion EGCG can alleviate intestine ischemia-reperfusion injury by suppressing inflammation and apoptosis,this protective effect may be mediated by suppressing Wnt/β-catenin signal pathway.
【CateGory Index】: R574
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