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《Chongqing Medicine》 2017-20
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Helicobacter pylori combined with MNU gavage for preparing balb/c mouse gastric cancer model

Sun Yanzhen;Zhang Tao;Chen Liangrong;Ou Chao;Huang Xiaoyan;Yang Jie;Cheng Yuanneng;Department of Gastroenterology,Affiliated Ruikang Hospital,Guangxi University of Chinese Medicine;Guangxi Zhuang Autonomous Region Institute of Tumor Prevention and Control;First Affiliated Hospital,Guangxi University of Chinese Medicine;  
Objective To investigate Helicobacter pylori combined with N-methyl-N-nitrosourea(MNU)gavage for preparing balb/c mouse gastric cancer model.Methods Eighty balb/c mice were randomly divided into four groups after 1-week adaptive feed,normal group,model group Ⅰ,Ⅱ and Ⅲ,20 cases in each group.The model groupⅠ,Ⅱ andⅢ were given Helicobacter pylori bacteria liquid(CFU=109/mL)gavage,once every other day for 5times;then,the freshly configured MNU solution 0.15,0.3,0.6mL gavages were in turn given,MNU and pure water allocation ratio was 5mg:3mL.Once gavage per week for continuous 10 weeks.Results The model group II had 66.67% adenocarcinoma,the model group I were gastritis with mild atypical hyperplasia,and all mice in the model group III died.Conclusion This method can prepare the gastric cancer model.
【Fund】: 国家自然科学基金资助项目(81360531);; 广西卫生厅重点项目(重2012030)
【CateGory Index】: R-332;R735.2
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