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《Chongqing Medicine》 2017-21
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Effects comparison of chemical hypoxia induced by cobalt chloride and hypoxia environment on rat pulmonary arterial fibroblasts

Chai Xiaoyu;Xu Huiying;Liu Zhonghui;Yi Liang;Liu Xinmin;Department of Geriatrics,First Hospital,Peking University;  
Objective To compare the effects of cobalt chloride(CoCl_2)induced chemical hypoxia and hypoxia environment on the proliferation,migration and phenotype transformation of rat primary pulmonary artery fibroblasts(PAFs).Methods Primary PAFs were isolated and cultured.Cells were stimulated by CoCl_2,or hypoxia cell culture(1% O_2)was used to stimulate and induce PAFs.Then the effects of CoCI2 and hypoxia environment on PAFs were compared by CCK-8 assay,scratch assay,transwell assay,phenotype marker protein expression and PI3K/Akt signaling pathway protein expression.Results Compared with the control group,100 μmol/ mL CoCl_2 stimulation had no significant effect on the cell proliferation activity,cell migration ability and phenotype transformation ability of PAFs(P0.05);while 1% O_2 could significantly improve the cell proliferation and migration activities of PAFs as well as the upregulation ofα-SMA expression(P0.05).Conclusion There exist differences of effects between CoCl_2 induced chemical hypoxia and hypoxia environment on promoting cell proliferation,cell migration and phenotype transformation in PAFs.
【Fund】: 国家自然科学基金资助项目(81270114)
【CateGory Index】: R543.2
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Georgina N.Masoud;Wei Li;Department of Pharmaceutical Sciences, College of Pharmacy, the University of Tennessee Health Science Center;;HIF-1α pathway: role, regulation and intervention for cancer therapy[J];Acta Pharmaceutica Sinica B;2015-05
【Co-citations】
Chinese Journal Full-text Database 9 Hits
1 Chai Xiaoyu;Xu Huiying;Liu Zhonghui;Yi Liang;Liu Xinmin;Department of Geriatrics,First Hospital,Peking University;;Effects comparison of chemical hypoxia induced by cobalt chloride and hypoxia environment on rat pulmonary arterial fibroblasts[J];重庆医学;2017-21
2 HU Bing;AN Hong-Mei;WANG Shuang-Shuang;Department of Oncology,Longhua Hospital,Shanghai University of Traditional Chinese Medicine;;Effects and Mechanism of YangGan JieDu SanJie Herbs on Angiogenesis in Hepatocellular Carcinoma[J];中西医结合肝病杂志;2017-03
3 DING Kaiqiang;TIAN Wei;ZHOU Dewang;FENG Shuang;YANG Yong;Drug Research Institute,China Pharmaceutical University;;Advances in the research and development of HIF-1 inhibitors[J];药学研究;2017-06
4 CHAI Xiao-yu;XU Hui-ying;LIU Zhong-hui;YI Liang;LIU Xin-min;Geriatrics of Peking University First Hospital;;Effect of Cobalt Chloride on Pulmonary Arterial Fibroblasts of Rat[J];现代生物医学进展;2017-15
5 WANG Chengqiang;HE Shengmao;XIE Xiaobo;LI Qi;Department ofOrthopedics,Zhujiang Hospital of Southern Medical Hospital;;Suppression of RANKL/OPG pathway activation in FLSs in interfacial membrane through up-regulation of HIF-1α induced by Ti particles[J];实用医学杂志;2017-10
6 YU Tianchi;TANG Bo;Department of Vascular Surgery,the First Affiliated Hospital of Harbin Medical University;;Development of Inhibitors Targeting Hypoxia-inducible Factor-1 and Hypoxia-inducible Factor-2 for Cancer Therapy[J];医学综述;2017-07
7 ZHANG Zhihui;WANG Lili;GAO Hua;ZHANG Jian;LI Juan;LI Yuan;WU Chunxiao;LU Zhiming;Department of Clinical Laboratory,Shandong Provincial Hospital Affiliated to Shandong University;;Role of hypoxia-inducible factor-1α in the regulation of programmed death ligand 1 expression in lung adenocarcinoma[J];山东大学学报(医学版);2017-04
8 Xiang-li Wang;Key Laboratory of Nanobiological Technology of Chinese Ministry of Health, Xiangya Hospital of Central South University;;Hypoxia regulates glycolysis and metastasis of prostatic cancer in vitro[J];中国现代医学杂志;2016-23
9 XIONG Wei-peng;Division of Endocrinology,Department of Medicine,University of Colorado School of Medicine;;Role of hypoxia-inducible factor-1 in immune escape of tumor cells[J];解剖学报;2016-06
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