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《Chongqing Medicine》 2017-21
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Effects comparison of chemical hypoxia induced by cobalt chloride and hypoxia environment on rat pulmonary arterial fibroblasts

Chai Xiaoyu;Xu Huiying;Liu Zhonghui;Yi Liang;Liu Xinmin;Department of Geriatrics,First Hospital,Peking University;  
Objective To compare the effects of cobalt chloride(CoCl_2)induced chemical hypoxia and hypoxia environment on the proliferation,migration and phenotype transformation of rat primary pulmonary artery fibroblasts(PAFs).Methods Primary PAFs were isolated and cultured.Cells were stimulated by CoCl_2,or hypoxia cell culture(1% O_2)was used to stimulate and induce PAFs.Then the effects of CoCI2 and hypoxia environment on PAFs were compared by CCK-8 assay,scratch assay,transwell assay,phenotype marker protein expression and PI3K/Akt signaling pathway protein expression.Results Compared with the control group,100 μmol/ mL CoCl_2 stimulation had no significant effect on the cell proliferation activity,cell migration ability and phenotype transformation ability of PAFs(P0.05);while 1% O_2 could significantly improve the cell proliferation and migration activities of PAFs as well as the upregulation ofα-SMA expression(P0.05).Conclusion There exist differences of effects between CoCl_2 induced chemical hypoxia and hypoxia environment on promoting cell proliferation,cell migration and phenotype transformation in PAFs.
【Fund】: 国家自然科学基金资助项目(81270114)
【CateGory Index】: R543.2
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