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《Chongqing Medicine》 2017-21
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Effects of down-regulation of PI3K/Akt signaling pathway on growth of laryngeal cancer cells

Wang Huimin;Yu Wenfa;Zhou Hang;Lu Zhenmin;Department of Otorhinolaryngology,First Affiliated Hospital of Xinxiang Medical University;  
Objective To investigate the effect of potassium channel blocker tetraethtylamine(TEA)on the proliferation and apoptosis of human laryngeal carcinoma cells(Hep-2)through the PI3K/Akt signaling pathway.Methods TEA acted on HEp-2.The methyl thiazolyl blue(MTT)method was used to determine the Hep-2 cell activity and the proliferation inhibition rate of TEA on Hep-2 was calculated;the Hoechest 33258 staining was used to detect cell apoptosis, and the Hep-2 apoptosis rate was detected by flow cytometry(FCM)assay.Results After inoculation by 5,10,20 mmol/L TEA,the Hep-2 cell proliferation inhibition rate reached 12.573% ,31.385% and 56.132% ,respectively.After 96 h Hep-2 cell action,the cellular apoptosis rates were(41.64±2.67)% ,(58.76±4.32)% and(72.65±6.54)% respectively,the inhibition rate and apoptosis rate in the TEA treating group were significantly higher than those in the non-TEA treating group,the differences were statistically significant(P0.05).Conclusion TEA can inhibit the proliferation and in vivo growth of human laryngeal carcinoma cells and promotes the apoptosis of laryngeal cancer cells.
【Fund】: 河南省重点科技攻关项目(152102310355)
【CateGory Index】: R739.65
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【Citations】
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【Co-citations】
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