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《Chongqing Medicine》 2017-34
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Study on the effect ofβ-TCP/PLGA scaffolds seeded BMSCs combing with hyperbaric Oxygen on bone defect with seawater immersing in rabbit radius

Zhang Gan;Cheng Xunsheng;Chen Xiaosong;Ma Wuxiu;Chen Congcong;Fu Laixiang;Department of Orthopedics,the 105th Hospital of Chinese People′s Liberation Army;  
Objective To investigate therapeutic effects ofβ-TCP/PLGA scaffolds seeded bone marrow mesenchymal stem cells(BMSCs)combing with hyperbaric oxygen(HBO)on bone defect with seawater immersing in rabbit radius.Methods BMSCs were seeded into theβ-TCP/PLGA scaffolds to construct tissue engineering bone.60 New Zealand rabbits were created 1.5 cm bone defect in bilateral radius,and then bilateral wound limbs were immersed in seawater for 3 h.After debridement,all rabbits were randomly divided into 4 groups.In group A nothing was implanted into the bone defect.Only BMSCs was implanted in group B.BMSCs+HBO was given in group C.β-TCP/PLGA BMSCs+HBO was given in group D.At postoperative 4,8,12 weeks rabbits were sacrificed after radiography radius.X-ray radiography,HE staining,immunohistochemical observation were used to evaluate repairing effect of bone defect with seawater immersing.Results Radiographic analysis demonstrated that the bone defects were completely repaired with recanalization of the medullary cavity in group D,bone defects were partially repaired with partial recanalization of the medullary cavity in group C,bone defects were incompletely repaired in group B;bone defect repair unfinished and the broken ends were sclerous in group A.Each time point group Dgroup Cgroup Bgroup A in callus grey value(P0.05).HE staining indicated after 12 weeks,a small amount of lamellar bone formated in group A;a small amount of lamellar bone formated in group B;a large amount of lamellar bone formated in group C;a large amount of lamellar bone formated in group D.Immunohistoehemical result indicated at postoperative 4 weeks,the expressions of osteocalcin(OCN)in each group were at high levels,the expressions decreased significantly at postoperative 8 weeks,the expressions were at a low level at postoperative 12 weeks.At postoperative 4 and 8 weeks,the expression levels of OCN were group Dgroup Cgroup Bgroup A(P0.05),there was no significant difference at postoperative 12 weeks between the 4 groups(P0.05).Conclusion theβ-TCP/PLGA composite BMSCs combined hyperbaric oxygen is the effective method to repair seawater immersed bone defect in rabbit.
【Fund】: 南京军区医学科技创新重点课题(11Z011)
【CateGory Index】: R687.3
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