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《Chongqing Medicine》 2017-34
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Effects of low temperature compound propofol on expression and ultrastructural changes of hippocampal neurons apoptosis proteinin rats

He Ya;Liu Xiaoying;Deng Qinghua;Zheng Xiaohong;Jiang Hongyan;Su Yuanqi;Peng Lan;Department of Anesthesiology,the People′s Hospital in Yubei District of Chongqing city;Chongqing Medical and Pharmaceutical College;  
Objective To observe the effect of low temperature compound propofol on the changes of apoptosis protein Caspase-3,autophagy-related proteins Beclin-1 and LC3-Ⅱand thier changes of hippocampal neurons.Methods The rats were randomly divided into blank control group(Group A),propofol group at low temperature(Group B)and chloral hydrate group at low temperature(Group C),Group B and C were treated with low temperature for 30 min.Then,each group was subjected to cardiac perfusion and decapitated brain to prepare rat hippocampal neuronal tissue samples.The expression of Caspase-3 was detected by immunohistochemistry,Beclin-1 and LC3-Ⅱ protein was detected by immunoblotting.The ultrastructural changes of neurons were observed by transmission electron microscopy.Results The expression of Caspase-3,Beclin-1 and LC3-Ⅱ protein in each group were statistically significant differences(P0.05).The results of transmission electron microscopy showed that the neurons in group B and C were changed in different degrees,especially in group C neuronal apoptosis is obvious.Conclusion Autophagy and apoptosis in existence still exist in low temperature condition,while propofol can reduce this damage and have better protective effect on neurons.
【Fund】: 重庆市教委科学技术研究项目(KJ112502)
【CateGory Index】: R614
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