Lithium chloride maintains the gap junction of hypoxic myocardium by inhibiting glycogen synthase kinase 3β
Zhou Yang;Liu Lingxi;Zhao Fei;Tang Shihai;Xiao Yingbin;Peng Huali;Department of Cardiothoracic Surgery,the People′s Hospital of Leshan city;Department of Cardiovascular Surgery,Xinqiao Hospital,the Third Military Medical University;
Objective To study the effect of lithium chloride on the gap junction in the myocardium under chronic hypoxia.Methods Twenty-five C57 BL/6 Jmice were randomly divided into normoxia group,hypoxia group,normoxic control group,hypoxia+ saline group and hypoxia+lithium chloride group.Hypoxia group was treated with 10% oxygen concentration for 4 weeks.Hypoxia+ saline group and hypoxia + lithium chloride group were intraperitoneal injection of saline and lithium chloride.Electrophysiology and cardiac catheterization were used to assess arrhythmias,heart rate and ejection fraction.The expression of Cx43,phosphorylated glycogen synthase kinase 3β(p-GSK-3β)and glycogen synthase kinase 3β(GSK-3β)were detected by Western blot.Results Compared with the normoxia group,the hypoxia group had a faster heart rate[(448 ± 18)bpmvs.(401 ± 13)bpm,P0.05),and the ejection fraction was decreased[(56±5)%vs.73±4)%,P0.05],arrhythmia score increased[(3.4±0.5)%vs.(0.6±0.5)%,P0.05],Cx43 expression was decreased.Compared to hypoxia + normal saline group,the heart rate decreased[(412±11)bpmvs.(454±18)bpm,P0.05],ejection fraction increased[(69±3)% vs.(55±4)%,P0.05],the score of arrhythmia decreased[(1.8±0.4)%vs.(3.0±0.7)%,P0.05]in hypoxia+lithium chloride group,the expression of Cx43 and the rate of p-GSK-3βto GSK-3βwere increased.Conclusion During the chronic hypoxia,lithium chloride can sustain the gap junction through inhibition of GSK-3βsignaling way,which can also reduce the rate of arrhythmia.
【CateGory Index】： R541.7