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《Journal of Agricultural University of Hebei》 2001-02
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Late blight resistant transgenic potato expressing glucose oxidase gene

ZHANG Li ping 1, YANG Jing hua 1, LI Tian ran 1, YAO Yu qi 2, ZHANG He ling 1 (1.Faculity of Life Sciences, Inner Mongolia University, Huhhot 010021, China; 2.Inner Mongolia Academy of Agricultural Sciences, Huhhot 010031, China)  
Two commercial potato cultivars “Atlantic" and “Shepody" were transformed with glucose oxidase gene from Aspergillus niger via Agrobacterium tumefaciens mediated gene transfer. 23 regenerated transgenic potato plants were obtained. Kanamycin resistance, PCR amplification, nucleic acid spot hybridization, southern blot analysis showed that the glucose oxidase gene was intergrated into transformed potato genome. Southern blot analysis revealed that there are 2~4 copies of glucose oxidase gene in each potato tetraploid genome. Detached transgenic potato leaves were inoculated with spors of Phytophthora infestans . Tests show that the number and the size of lesions were reduced and the appearance of lesions was postponed on the leaves of some transgenic lines.
【CateGory Index】: S435.32
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【References】
Chinese Journal Full-text Database 8 Hits
1 Huang Xianqun 1 * Jiang Minhua 1 Chen Jian 2, 3 Zhu Zhenyu 1 Li Li 1 Lin Zhongping 2 1 Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Institute of Biotechnology, Guiyang, 550006; 2 College of Life Science, Peking University, Beijing, 100816; 3 Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou, 350003;Integrating the hrap Gene from Sweet Pepper into Potato Enhances Resistance to Phytopthora infestans[J];Molecular Plant Breeding;2011-02
2 JIN Guang-hui, WEN Jing-zhi ,DING Guang-zhou(Potato Research Institute of Heilongjiang Academy of Agricultural Sciences, Keshan 161606);Suggest and Historical and Current Studies of Potato Late Blight in China[J];Heilongjiang Agricultural Science;2002-06
3 ZHU He-qin,FENG Zi-li,LI Zhi-fang,ZHAO Li-hong,SHI Yong-qiang,YIN Zhi-xin(Cotton Research Institute of CAAS/Key Laboratory of Cotton Genetics Improvement,MOA,Anyang,Henan 455000,China);Verticillium Wilt Resistance of A Transgenic Cotton Line with Chitinase and Glucanase Genes[J];Cotton Science;2011-01
4 PENG Xin-qin DIAO Wen-yi HUO Miao-juan XIONG Xin-hua WANG Xiang-hui HU Jia-jin (College of Bioscience and Biotechnology of Hunan Agricultural University,Changsha,China 410128) (Crop gene Engineering key Laboratory of Hunan Province,Changsha,China 410128);Progress on Gene Engeneering in Late Blight Disease of Potato[J];Progress in Modern Biomedicine;2007-01
5 Jian Guiliang Liu Huijun Lu Meiguang(Institute of Plant Protection,CAAS, The State Key Lab. for Biology of Plant Diseases and Insect Pest, Beijing 100094);Expression of Glucose Oxidase in Transfer GO Cotton[J];Biotechnology Bulletin;2006-S1
6 LI Xian ping 1,HE Yun kun 1,CHEN Shan na 2, ZHAO Zhi jian 1,LI Shu lian 3,LI Ru gang 4 ( 1. Biotechnology Research Institute, Yunnan Academy of Agricultural Sciences,Kunming 650223 China; 2. Biology Department, Yunnan University,Kunming 650;Analysis of Late Blight Resistance in Transgenic Potato Expressing Harpin_(Ea) Gene[J];Journal of Yunnan Agricultural University;2002-03
7 HE Chuan-yun1,SHAO Ji-rong1,ZHU Xue-mei2,XIE Rong3,BAI Da-quan1,LIU Cheng-yuan3(1.College of Biology and Science,Sichuan Agricultural University,Ya'an 625014,China;2.College of Resources and Environmental,Sichuan Agricultural University,Ya'an 625014,China;3.Rice and Sorghum Research Institute,Sichuan Academy of Agricultural Sciences,Luzhou 646000,China);Introducing Glucose Oxidase Gene into O. sativa ssp. Indica from Transgenic Oryza sativa L. ssp. japonica Utilizing Sexual Hybridization[J];Seed;2007-11
8 HUANG Xianqun1,JIANG Minhua1,CHEN Jian2,3,ZHU Zhenyu1,LI Li1 and LIN Zhongping2(1.Guizhou Institute of Biotechnology,Guizhou Key Laboratory of Agricultural Biotechnology,Guiyang 550006,China;2.Beijing University,College of Life Science,Beijing 100816,China;3.Institue of Biotechnology,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China);Studies on Transformation hrap Gene into Potato by Agrobacterium-mediated[J];Acta Agriculturae Boreali-Occidentalis Sinica;2010-11
China Proceedings of conference Full-text Database 1 Hits
1 ZHANG Helinb~1 YA Huiyuan~1 ZHANG Limei~2 CHAO Xianwei~2 ZHA NG Jianfeng~3 JIA NG Bowei~1 ZHA NG Ruofang~1 1. College of Life Sciences Inner Mongolia University, Huhhot 010021, China; 2. South China Agricultural University, Guangzhou 510642, China; 3. Inner Mongolia Sugar Industry Institute Huhhot 010070, China;Comparative Study on the Glucose Oxidase Genes of Aspergillus niger 3.758 and 3.324[A];[C];2008
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 FENG Pei-yong et al(College of Life Science,Ludong University,Yantai,Shandong 264025);Study on the Property of Producing Cellulase by a Strain of Aspergillus niger[J];Journal of Anhui Agricultural Sciences;2007-36
2 Zhang Heling; Song Yanru; Pen Xuexian;Li Tianran; Meng Qing; Cui Xiaojiang; Hou Linlin; Zhang Lizu; Ma Qing huThe transgenic potato cultivars ″Tiger head ″and Kexin No. 4 transformed with both PVX and PVY coat protein genes were mechanically inoculated;ENGINEERING RESISTANCE TO MIXED PVX AND PVY INFECTION IN POTATO CULTIVARS EXPRESSING BOTH PVX AND PVY COAT PROTEIN GENES[J];Chinese Journal of Virology;1996-04
3 LU Rui-fang, LU Peng-fei, PENG Xue-xian (Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China);RNA-mediated Relatively Broad Resistance of Potato Y Virus NIb Replicase Gene[J];CHINESE JOURNAL OF VIROLOGY;2000-02
4 DENG Wen sheng, YANG Xi cai, KANG Liang yi, TIEN Po (Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China);Gene Construction and Activity Detection of the Ribozyme Multi-targeting against Negative Strand of Potato Spindle Tuber Viroid in vitro[J];Chinese Journal of Virology;2000-04
5 Zhang Heling Li Tianran Hasi Agula Er Dun Zhang Tong Sun Yan Pang Ruijie(Department of Biology, Inner Mongolia Univeristy, Huhehaote100021);RESISSTANCE OF TRANSGENIC FOTATO CULTIVARS TOPOTATO LEAFROLL VIRUS[J];CHINESE JOURNAL OF VIROLOGY;1995-04
6 Yang Jinghua Hasi Agula Liang Chenggang Zhang Heling (Biology departemnt,Inner Mongolia University,Huhehaote,China,010021);CLEAVAGE OF NEGATIVE STRAND RNA OF POTATO LEAFROLL VIRUS REPLICASE GENE IN VITRO BY RIBOZYME[J];CHINESE JOURNAL OF VIROLOGY;1998-02
7 LIU Jing-ping1,LI Jin1,GE Xing2(1.Teachers' College of Beijing Union University,Beijing 100011,China;2.Department of Basic Science,Beijing University of Agriculture,Beijing 102206,China);Using the Enzyme-Inhibited Reaction Method to Detect Cd,Sn and Pb ions Residuals in Foods[J];Journal of Beijing University of Agriculture;2007-04
8 ZHAN JiXun,LIU TingZhi,TIAN ShengYan,WANG DePei,DING YouFang(School of Food Science and bioengineering,Tianjin University of Science and Technology, Tianjin 300222, China);STUDIES ON PROPERTIES AND APPLICATIONS OF XYLANASE PRODUCED BY ASPERGILLUS NIGER S13[J];Journal of Beijing Technology and Business University (Natural Science Edition);2003-02
9 Jia Yingmin(Dept. of Food Science,Hebei Agric. Univ.,Baoding 071001 )Zhao Xuehui(Dept. of Food Science and Technology,Hua Zhong Agric. Univ-,Wuhan 430070);Production and Properties of Inulinase From Aspergillus niger M_(89)[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1997-01
10 Zhu Jiehua; Zhang Zhiming; Yiang zhihui (Department of Plant Pathology, Hebei Agricultural University, Baoding 071001);Review of A2 Mating Type of Potato Late Blight Pathogen (Phytophthora infestans)[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1999-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 CHEN Di-xin et al (College of Forestry,Henan University of Science and Technology,Luoyang,Henan 471003);Research Progress on Strawberry Resistance to Aphids and the Related Aphid-spread Virus Disease[J];Journal of Anhui Agricultural Sciences;2008-31
2 WANG Jianan1, LV Wenhe1, JIN Guanghui2, BAI Yamei3, LI Wenxia1, HAN Yingpeng1 (1. College of Agriculture, Northeast Agricultural University, Harbin 150030, China; 2. College of Agriculture, Heilongjiang August First Land Reclamation University, Daqing Heilongjiang 163319, China; 3. College of Resource and Environment, Northeast Agricultural University, Harbin 150030, China);Optimization of PCR system in EST-SSR analysis of Phytophthora infestans[J];Journal of Northeast Agricultural University;2009-06
3 CHENG Zhi-hui,XING Yu-jun(College of Horticulture,Northwest Sci-Tech University of Agriculture and Forestry, Yangling,Shaanxi 712100,China);In vitro Screening of Resistant Potato Clones to Late Blight with Crude Phytophthora infestans Toxin[J];Acta Botanica Boreali-occidentalia Sinica;2005-12
4 ZHAN Jin-bi1,JIANG Jian1,SHI Sheng-jun2,FENG Xue-song1,LIU Xia1,WU Shi-ping3,HE Hai-yong3(1.Meitan Station of Plant Protection and Quarantine,Meitan,Guizhou 564100;2.Huangjiaba Station for Popularizing Agricultural Technique,Meitan,Guizhou 564100;3.Guizhou Institute of Plant Protection,Guiyang,Guizhou 550006,China);Preliminary Identification of Late Blight Resistance of Some Main Cultivation Potato Varieties in Field[J];Guizhou Agricultural Sciences;2009-03
5 LI Mei1,BI Yang2,GE Yong-hong2,DENG Jian-jun1, XIE Dong-feng1 (1.College ofAgronomy, GansuAgricultural University, Gansu, Lanzhou 730070;2. College of Food Science and Engineering, Gansu Agricultural University, Gansu, Lanzhou 730070, China);Effect of preharvest Harpin treatment on latent infection of muskmelon cultivar Yindi[J];Journal of Gansu Agricultural University;2005-02
6 WANG Xiao-yu,CHEN Zhi-yi(Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China);Application and Research Development of Gene Resource in Anti-disease Gene Engineering[J];Acta Botanica Boreali-Occidentalia Sinica;2009-12
7 REN Xiu-yan (College of Life Science,Langfang Teacher College,Langfang,Hebei 065000);Progress Research on Cucumber by Harpins[J];Journal of Anhui Agricultural Sciences;2010-22
8 Huang Xianqun 1 * Jiang Minhua 1 Chen Jian 2, 3 Zhu Zhenyu 1 Li Li 1 Lin Zhongping 2 1 Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Institute of Biotechnology, Guiyang, 550006; 2 College of Life Science, Peking University, Beijing, 100816; 3 Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou, 350003;Integrating the hrap Gene from Sweet Pepper into Potato Enhances Resistance to Phytopthora infestans[J];Molecular Plant Breeding;2011-02
9 HUI Jiao,JIANG Ji-zhi,BAO Xue,WANG Fang(College of Life Sciences,Hebei University,Baoding 071002,China);Optimization of fermentation conditions of Streptomyces albus NB8 against the growth of Phytophthora infestans[J];Journal of Agricultural University of Hebei;2011-02
10 ZHENG Wen,ZHENG Xiu-ying;Study on the Control Efficiency of 25% Amistar on Potatoes Early Blight and Late Blight[J];Journal of Heilongjiang August First Land Reclamation University;2007-03
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