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A Study of the Biological Charactor of Bovine-RV Culture in Three Cell Iines

Sun Jiguo;Zheng Shixue;Liu Meihong(Dep.of Vet.Med, Hebei Agric, Uni, Baoding 071001)Zhao Donggang (Bureau. of the Animal Science and Aquatic Products, Baoding 071001 )  
In this study, the Bovine-Rv has been cultured with three cell lines (MA-104 lineand LLc-Mk2line and Hela line) and the biological charactors have been compared amongthese three cell lines: The result is that Bovine RV CAN GROW ON MA-104 with its growthposition being in its cytoplasm; After infected with Bovine Rv, obvious cytopathic effects(cpe) appear on MA-104 Cells. Bovine Rv can grow on LLc-Mk2Cells and the growth position of Bovine Rv of LLc-Mk2Cells is also in the cytoplasm,The more generation is Cultured,The more antigent cells oF Bovine Rv can be detected by IFA and EIA; when the Bovine Rvhas increased to a certain degree in the LLc-Mk2cells, The number of cells of Bovine Rv become stable, But cytopathic effects (cpe)cannot appear on the LLc-Mk2Cells infected withBovine Rv; Bovine Rv cannot grow in Hela cells.
【CateGory Index】: S852.6
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