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Electrofusion of Blastomeres From Mouse 2-And 4-Cell Embryos

Liu Sujuan(Department of Animal and Veterinary Science, Agricultural University of Hebei, Baoding 071001 )Zhang Yong(Department of Veterinary Medicine, North Western Agricultural University, Yang Ling 712100)  
The fusion rates of .mouse blastomeres from 2-cell embryo were 84.6%(33/39),100.0% (59/59),97.4% (74/76), 95. 1% (39/41), 64.7% (11/17) respectively, When embryos were treated at such electrofusion parameters that is one 800v/cm,from 40 to 80μsDC pulse,two1000v/cm,from 160 to 320us DC pulse and one 160-320μs pulse of 800,1000, 1200v/cm,and its development rates of 2-cell embryo were 15. 2 % (5/33),39.0%(23/59),47.3%(35/74),43.6% (17/39),18.2% (2/11) respectively. When the embryos were recovered at the time of 39~43 hours or 44~ 48 hours after LH injection and were treated with one 1000v/cm, DC pulse of 160~320μs, the fusion rates were 100.0% (27/27),96.4% (27/28),and its developmental rates were 44.4%(12/27),33.3% (9/27) respectively.The effectual electrofusion parameters were one 1000v/cm from 160 to 320μs DC pulse.
【CateGory Index】: Q954.45
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