Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Pratacultural Science》 2017-12
Add to Favorite Get Latest Update

Comparison of methods for detecting aflatoxins B_1 and M_1 in milk

Luo Yu-fen;Weng Xiu-xiu;Tang De-fu;Pan Jian-zhong;Nian Fang;Faculty of Science,Gansu Agricultural University;College of Pastoral Agriculture Science and Technology,Lanzhou University;Faculty of Animal Science and Technology,Gansu Agricultural University;Lanzhou Food and Drug Inspection;  
Immunoaffinity column-high performance liquid chromatography(HPLC)and enzyme-linked immunosorbent assay(ELISA)methods were adopted to determine aflatoxins(AF)B1 and M1 in milk.The recovery,detection limit,and precision of these two methods were compared in this study.The results showed that,for HPLC,the detection limits of AFB1 and AFM1 were 0.02 and 0.01μg·L-1,respectively,while for ELISA,the detection limits of AFB1 and AFM1 were 0.05 and 0.02μg·L~(-1),respectively.Standard solutions of AFB1(0.003,0.006,0.012,and 0.024μg·kg~(-1))and AFM1(0.005,0.01,0.025,and 0.05μg·kg~(-1))were added into blank milk samples and the results of recovery experiments indicated that,for HPLC,the recoveries of AFB1 and AFM1 were 77.58%~82.81% and 77.51%~82.23% with relative standard deviations of 1.93%and 3.51%,respectively.For ELISA,the recoveries of AFB1 and AFM1 were 77.28%~79.11% and 75.40%~76.34% with relative standard deviations of 2.20%and 3.80%,respectively.The recovery of AFB1 did not significantly differ between the two methods(P0.05).However,the recovery of AFM1,when the amount added was more than 0.025μg·kg~(-1),was significantly higher by HPLC than by ELISA(P0.05).In conclusion,both the tested methods were highly accurate,with a high sensitivity and good repeatability.At higher concentrations of AFM1,the accuracy of HPLC was better than that of ELISA.
【Fund】: 甘肃省自然科学基金(145RJZA058);; 兰州大学中央高校基本科研业务费专项资金(lzujbky-2014-198)
【CateGory Index】: TS252.7
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved