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《Acta Prataculturae Sinica》 2009-01
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Hybrid identification of progenies of Zoysia crosses by SRAP marker

XUE Dan-dan1,2,GUO Hai-lin1,ZHENG Yi-qi1,CHEN Xuan1,LIU Jian-xiu1(1.Insititute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China;2.College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China)  
Sequence-related Amplified Polymorphism(SRAP) is a new molecular marker technique developed on the basis of PCR.The authenticity of 37 progenies of 5 Zoysia grass cross-combinations were identified by SRAP marker to select out the true hybrids for further study.First,primers were selected to bands specific for the male parent but absent from the female,then 9 primers were selected and applied to identify the hybrids.The authenticity of the progenies was identified by whether or not they had the male parent specific bands.There were 32 progenies with these specific bands,which were therefore identified as true hybrids.The amplified bands of SRAP were clear and stable,and there was considerable variation in the hybrids,including some with new bands and some without the parent bands.The results suggest that SRAP markers could be used as effective molecular markers for the authenticity,identification and genetic analysis of the progenies of Zoysia grass
【Fund】: 江苏省科技攻关项目(BE2006339);; 国家科技支撑项目(2006BAD01A19-3);; 国家自然科学基金项目(30571307);; 江苏省高技术项目(BG2006320)资助
【CateGory Index】: S688.4
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