Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Prataculturae Sinica》 2011-04
Add to Favorite Get Latest Update

Optimization of SRAP-PCR system and its application in genetic diversity analysis of Stylosanthes

ZHANG Wei-li1,LIU Feng-min2,LIU Ai3(1.College of Life Sciences,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;2.Teaching and Science Reserch Base,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;3.College of Horticulture and Landscape Architecture,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)  
Two different genotypes of Stylosanthes germplasm,S.guianensis cv.Reyan No.2 and S.guianensis cv.Reyan No.13 were used as material for studying the effects of concentrations of Mg2+,dNTPs,DNA polymerase,primers and DNA template on the SRAP-PCR reactions and optimizing the establishment of SRAP molecular marker system in Stylosanthes.The optimum system was established as follows: template DNA 40 ng,Mg2+ 2.5 mmol/L,dNTPs 0.2 mmol/L,polymerase 1 U,primer 0.3 μmol/L,the total reaction volume was 25 μL.The genetic diversity and relationships of nine Stylosanthes germplasms were analyzed by using this optimum system.Forty-eight primers were studied for an analysis of genetic diversity by SRAP in Stylosanthes germplasms.Fourteen effective primers selected from 48 primers combination were used for SRAP-PCR,and 150 of the 154 DNA fragments amplified,showed polymorphisms.The average binds from each primer was 11.0 and the average percentage of polymorphic bands was 97.36%.The Nei's genetic similarity coefficient of the tested accessions ranged from 0.386 to 0.882 by software NTSYSpc 2.1 based on SRAP results,and the average Nei's coefficient was 0.631,average the genetic distance(GD) was 0.369.Based on the presence of bands,nine Stylosanthes germplasm were classified into there major groups by UPGMA cluster analysis,group Ⅰ,group Ⅱ and group Ⅲ.Group Ⅰ included S.hamata cv.Verano,and group Ⅱ included S.scabra cv.Seca.Group Ⅲ included S.guianensis cv.Reyan No.5,S.guianensis cv.Reyan No.10,S.guianensis cv.Reyan No.13,S.guianensis cv.White cook,S.guianensis cv.Reyan No.2,S.guianensis cv.Graham and S.guianensis tardio CIAT1283.This research should provide a scientific basis at the molecular level for further study and the application of nine Stylosanthes germplasms.The most of the varieties with relative relationship in their pedigrees and similar biological characteristics were clustered into the same group.
【Fund】: 广东省科技计划项目(2010B020305011)资助
【CateGory Index】: S541.9
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 10 Hits
1 BAI Chang-jun,LIU Guo-dao,YAO Qing-qun,YU Dao-geng,CHEN Zhi-quan(Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences(CATAS),Danzhou,Hainan Province 571737,China);Polymorphic Analysis of RAPD in Stylosanthes guianensis 'Reyan No.2' after Spaceship-carried Seed Induction[J];Acta Agrestia Sinica;2008-04
2 SUI Xiao-qing,WANG Kun (Institute of Grassland Science,College of Animal Science and Technology,China Agriculture University,Beijing 100094,China);Establishment and optimization of the ISSR-PCR reaction system in Stipa krylovii[J];Acta Prataculturae Sinica;2008-03
3 XUE Dan-dan1,2,ZHENG Yi-qi2,WANG Zhi-yong2,GUO Hai-lin2,CHEN Xuan2,LIU Jian-xiu2(1.College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;2.Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China);Establishment and optimization of SRAP-PCR reaction system for Zoysia[J];Acta Prataculturae Sinica;2008-06
4 TANG Yan-qiong1,HU Xin-wen1,GUO Jian-chun2,BAI Chang-jun3,HE Hua-xuan3(1.College of Agriculture,Hainan University,Danzhou 571737,China;2.Institute of Biotechnology and Bioscience,CATAS,Haikou 571101,China;3.Institute of Tropical Crops Genetic Resources,CATAS,Danzhou 571737,China);An analysis by ISSR of genetic diversities in Stylosanthes germplasms[J];Acta Prataculturae Sinica;2009-01
5 GUO Hai-lin1,2,ZHENG Yi-qi1,2,CHEN Xuan2,XUE Dan-dan1,2,LIU Jian-xiu2 (1.College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;2.Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China);Genetic diversity and relationships of zoysiagrass as revealed by SRAP markers[J];Acta Prataculturae Sinica;2009-05
6 YAN Jia-jun1,2,BAI Shi-qie1,2,ZHANG Xin-quan1,YOU Ming-hong2,ZHANG Chang-bing2,LI Da-xu2,ZENG Yi1(1.Department of Grassland,Sichuan Agricultural University,Ya'an 625014,China;2.Sichuan Grassland Science Academy,Chengdu 611731,China);Genetic diversity of wild Elymus sibiricus germplasm from the Qinghai-Tibetan Plateau in China detected by SRAP markers[J];Acta Prataculturae Sinica;2010-01
7 LING Yao1,2,ZHANG Xin-quan2,QI Xiao-fang2,ZHOU Ying-jie2,LIU Wei2,MA Xiao2,CHEN Shi-yong2 (1.College of Animal Medicine,Sichuan Agricultural University,Ya'an 625014,China;2.Department of Grassland,Sichuan Agricultural University,Ya'an 625014,China);Genetic diversity of wild Cynodon dactylon germplasm from five province of Southwest China and Africa detected by SRAP markers[J];Acta Prataculturae Sinica;2010-02
8 XIE Wen-gang,ZHANG Xin-quan,CHENG Yong-xia(Department of Grassland Science,Sichuan Agricultural University,Ya'an 625014,China);Identification and genetic variation analysis of orchardgrass hybrids(Dactylis glomerata)by SSR molecular markers[J];Acta Prataculturae Sinica;2010-02
9 LI Li,PENG Jian-ying~*,BAI Rui-xia,HAN Bin(College of Horticulture,Agricultural University of Hebei,Baoding,Hebei 071001,China);SRAP and TRAP Markers and their Application to Research about Horticultural Plants[J];Acta Botanica Boreali-Occidentalia Sinica;2006-08
10 LU Juan1,ZHANG Shao-ling1,LIU Qing-zhong2,WU Jun1,ZHANG Rui-ping1,LIAOYANG Wen-ke1,CHEN Ting1(1College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095 China;2Shandong Institute of Pomology,Tai'an,Shan-dong 271000 China);Optimization of SRAP-PCR system and its application in genetic diversity analysis of cherry[J];Journal of Fruit Science;2009-02
Chinese Journal Full-text Database 10 Hits
1 LI Xue-feng et al(Department of Pratacultural Science,College of Agronomy,Hainan University,Danzhou,Hainan 571737);Study on the Extraction Technology of Stylosanthes Leaf Protein Concentrate[J];Journal of Anhui Agricultural Sciences;2008-26
2 WEI Jia-shao, BAI Chang-jun, CAI Bi-yun (Tropical Pasture Research Center CATAS, Danzhou Hainan 571737,China);A Study on Dynamic Change of Forage Yield and Quality in Stylosanthes guianensis cv.Reyan No.5[J];Acta Agrestia Sinica;2001-03
3 YAN Lin-ling1,2,BAI Chang-jun2,LIU Guo-dao2(1.Agricultural College,Hainan University,Danzhou,Hainan Province 571737,China;2.Tropical Crop Genetic Resource Institute,CATAS,Danzhou,Hannan Province 571737,China);Multi-objective Decision on Seeking New Anthracnose Resistant Stylosanthes Accessions Induced by Space Flight[J];Acta Agrestia Sinica;2009-01
4 WANG Fang1,YUAN Qing-hua1,2*(1.College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou,Gansu Province 730020,China;2.Insfitute of Animal Science,CAAS,Beijing 100193,china);Establishment and Optimization of ISSR Reaction System for Agropyron cristatum(L.) Gaertn.[J];Acta Agrestia Sinica;2009-03
5 WANG Mi1,2,WEI Jian-min1,GUO Hui-qin1,REN Wei-bo2*,XU Zhu2,CHEN Li-bo2(1.Institute of Bioengineer Science,Inner Mongolia Agricultural University,Huhhot 010018,Inner Mongolia Autonomous Region,China; 2.Grassland Research Institute,CAAS,Huhhot 010010,Inner Mongolia Autonomous Region,China);Selection of Space Flight Mutation of Alfalfa and Its Polymorphic Analysis by RAPD[J];Acta Agrestia Sinica;2009-06
6 ZHOU Liang-Bin1,LU Xin-Shi1,LAI Li-li2 (1.Grassland Research Institute,Beijing Forestry University,Beijing 100083,China;2.College of Pratacultural and Environmental Sciences,Xinjiang Agricultural University,Urumchi,Xinjiang Province 830052,China);Optimization for SRAP-PCR System of Medicago varia Martin. Based on Orthogonal Design[J];Acta Agrestia Sinica;2010-03
7 JIANG Chang shun, ZOU Dong mei (Tropical Field Crops and Animal Husbandry Research Institute, Chinese Academy of Tropical Aricultural Sciences, Danzhou, Hainan, 571737);Investigaton on the Quality and Quantity of Stylosanthes guianensis cv. Reyan NO.2 Cultured on Hainan island[J];Grassland and Turf;2000-04
8 YANG Shui-lian,LIU Wei-dong,MA Tao,SHU Bi-chao,LIU Rui-feng,XIE Peng(Central South University of Forestry and Technology,Changsha 410004,China);Establishment and optimization of ISSR-PCR reaction system of centipedegrass[J];Grassland and Turf;2009-01
9 LIU Zhen1,2,LIU Jin-xiang2,ZHANG Shi-wei1,2(1.College of Pratacultural Science,Gansu Agricultural University,Lanzhou730070,China;2.Institute of Tropical Pratacultural Science,Zhanjiang Normal College,Zhanjiang 524048,China);Effect of defoliation on photosynthetic characteristics of Stylosanthes guianenesis cv. Reyan No.13[J];Grassland and Turf;2009-01
10 CHEN Li-qiang,SHI Shang-li,MAN Yuan-run (College of Pratacultural Science,Gansu Agricultural University,Key Laboratory of Grassland Ecosystem,Ministry of Education Sino-U.S.Centers for Grazingland Ecosystem Sustainability.Lanzhou 730070,China);Isozymes analysis of Longdong wild alfalfa[J];Grassland and Turf;2010-01
China Proceedings of conference Full-text Database 5 Hits
1 QIAO Yan-chun~(1,2),LIN Shun-quan~1,GUO Dong-liang~3 (1.College of Horticulture,South China Agricultural University,Guangzhou,Guangdong 510642,China; 2.Guang zhou Academy of Agricultural Sciences,Guangzhou,Guangdong 510308,China;3.Fruit Tree Research Institute,Guangdong Academy of Agricultural Science,Guangzhou,Guangdong 510641,China);The study of using SRAP markers in Genus Eriobotrya[A];[C];2010
2 Jiang Changshun~(1,2),Zou Dongmei~2,Liu Guodao~2,Zhang Yizheng~1 (1,Life Science College,Sichuan University,Chengdu,610064;2,Tropical Field Crops and Animal Husbandry Research Institute,CATAS,Hainan,571737,China);The Selection and Utilization of Stylosanthes guianensis cv.Reyan No.7[A];[C];2002
3 REN Wei-bo~1,WANG Mi~(1,2),CHEN Li-bo~1,XU Zhu~1 (1.Institute of Grassland Research,Chinese Academy of Agricultural Sciences,Huhhot 010010,China; 2.Institute of bioengineer science,Inner Mongolia agricultural university,Huhhot 010018,China);Progress of Mutation Induced by Space Flight Factors on Forages[A];[C];2009
4 ZHOU Ying-jie~1,Wang Xian-guo~(2*),ZHANG Xin-quan~1 (1.Department Grassland Science,Sichuan Agricultural University,Ya'an,625014,China; 2.Institute of Grassland Science,China Agricultural University,Beijing 100193,China;);Genetic Diversity of Buchloe dactyloides based on SRAP markers[A];[C];2010
5 Zhuang Nansheng, Wang ying, Tang yanqiong (College of Agronomy, South China University of Tropical Agriculture,Danzhou Hainan 571737);The Biological Effect of ultrasonic wave on Stylosanthes guianensis seeds[A];[C];2004
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Liu Jianxiu;He Shanan;Chen Shoaling(Institute of Boany, Jiangsu Province and Chinese Academy of Science, Nanjing 210014);Morphologicol type and turfgrass quality of zoysiagrass in East China[J];ACTA AGRESTIA SINICA;1997-01
2 Liu Jianxiu; Liu Yongdong; He Shanan; Chen Shouliang(Institute of Botany, Jiangsu Province & Chinese Academy, Naning 210014);Species Diversity of Chinese Warm Season Turfgrass and the Characteristic of Geography Distribution[J];ACTA AGRESTIA SINICA;1998-01
3 LIU Guo dao,BAI Chang jun,HE Hua xuan,WANG Dun jin,ZHOU Jia suo (Tropical Pasture Research Center CATAS,Danzhou Hainan 571737,China);The Selection and Utilization of Stylosanthes guianensis cv.Reyan No.5[J];Acta Agrestia Sinica;2001-01
4 LI Ya1, XIE Xiaojin2, XUAN Jiping1, LIU Jianxiu1(1.Institute of Botany of Jiangsu Province,Chinese Academy of Sciences,Nanjing,Jiangsu Province 210014,China;2.Nanjing Agricultural University,Nanjing,Jiangsu Province 210095,China);Assessment of Cold-Tolerance of China's Zoysia spp.[J];Acta Agrestia Sinica;2003-03
5 LI Ya, GENG Lei, LIU Jian-xiu(Institute of Botany, Chinese Academy of Sciences of Jiangsu Province,Nanjing,Jingsu Province 210014,China);Assessment on Salt-tolerance of Zoysia spp. in China[J];Acta Agrestia Sinica;2004-01
6 MAO Pei-sheng1,ZHANG Tao1,2,YANG Qing-chuan3 (1.Institute of Grassland Science,China Agriculture University,Beijing 100094,China;2.Department of Food Science,Tianjin Agriculture University,Tianjin 300384,China;3.Institute of Animal and Veterinary Science,CAAS,Beijing 100094,China);Study on the Verification of Alfalfa Cultivars by RAPD Molecular Markers[J];Acta Agrestia Sinica;2007-02
7 LIU Li1, TANG Feng-lan2, ZHANG Yue-xue2, HAN Wei-bo2, LIU Jie-lin2, HAO Ruo-chao2, XU Xiang-ling1, LI Ji-lin1 (1.Biology Department, Life and Environment Science College, Harbin Normal University, Harbin,Heilongjiang Province 150080, China; 2.Institute of Pratacultural Sciences, Heilongjiang Academy of Agricultural Sciences, Harbin,Heilongjiang Province 150086, China);Cytological Effects of Aerospace Flight and ~(60)Co-γ-Rays Irradiation on the Seeds of Lactuca indica L[J];Acta Agrestia Sinica;2007-05
8 ZHOU Zhi-fang1,2,XUAN Ji-ping2,LIU Jian-xiu2* (1.College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu Province 210095,China;2.Zhongshan Arboretum,Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing,Jiangsu Province 210014,China);Isozyme Study on Interspecies Genetic Relationship of Zoysia Willd.[J];Acta Agrestia Sinica;2007-06
9 ZHANG Xiao-ai, ZHANG Xin-quan, YANG Chun-hua,LIU Wei, GAN You-min(Department of Pratacultural Science,Sichuan Agricultural University, Ya'an 625014);General Conditions of Bermudagrass Germ Resources and Inheritance Diversity[J];Grassland and Turf;2003-04
10 ZHANG Xiao-ai~1,ZHANG Xin-quan~2 (1.Department of Horticulture,Sichuan Agricultural University,Ya'an 625014,China;2.Department of Grassland Science,College of Animal Science and Technology,Sichuan Agricultural University,Ya'an 625014,China);Study on the morphological characteristics of native wild Cynodon dactylon in southwestern region of China[J];Grassland and Turf;2006-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved