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《Acta Prataculturae Sinica》 2011-04
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Isolation and identification of four new Erwinia carotovora subsp.carotovora strains

ZHAO Ling1,2,3,4,CHAI Zhao-xiang1,LI Jin-hua1,2,3,4,WANG Di2(1.College of Grassland Sciences,Gansu Agricultural University,Lanzhou 730070,China;2.Gansu Key Lab of Crop Improvement and Germplasm Enhancement,Lanzhou 730070,China;3.Key Laboratory of Grassland Ecosystem,Ministry of Education,Lanzhou 730070,China;4.Sino-U.S.Centers for Grazingland Ecosystem Sustainability,Lanzhou 730070,China)  
Rhizosphere soils of potato(Solanum tuberosum) were sampled via a chess-board pattern from fields of potato grown for one year,continuously for 2 years,3 years and 4 years at 4 growing stages(before planting,squaring stage,maturity stage and harvest stage).The soils were isolated on CVP medium via the dilution method for soft rot Erwinia and 42 strains were obtained.During observations of morphology and tests of their physiological and biochemical characteristics,it was found that 4 of the 42 strains shared the same characteristics but differed from the other strains.The 4 strains were GAUP-b410,GAUP-b492,GAUP-b416 and GAUP-d34.The pathogenicity tests indicated that these 4 strains were artificially pathogenetic to cut potato pieces.GAUP-b410 and GAUP-b492 were taken at random for analysis of 16S rDNA sequence,which showed 99% sequence identity with 18 E.carotovora subsp.carotovora strains from Chinese cabbage in China after aligning data in GenBank.Microsoft DNAStar was used to draw the phylogenetic tree of strain GAUP-b410 and GAUP-b492.The tree showed that strain GAUP-b410 was in the same cluster as the 18 Chinese strains,while strain GAUP-b492 was in the same cluster as the 16 of 18 Chinese strains.Therefore,based on the physiological and biochemical characteristics of the 4 strains and the result of 16S rDNA sequence analysis of the 2 strains,the 4 strains were identified as the E.carotovora subsp.carotovora,which were 4 new E.carotovora subsp.carotovora strains.
【Fund】: 甘肃省农业生物技术研究与应用开发项目(GNSW-2006-01);; 现代农业产业技术体系建设专项资金(CARS-10-P18);; 国家科技支撑计划项目(2006BAD21B05)资助
【CateGory Index】: S435.32
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