Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Prataculturae Sinica》 2011-04
Add to Favorite Get Latest Update

Isolation and identification of four new Erwinia carotovora subsp.carotovora strains

ZHAO Ling1,2,3,4,CHAI Zhao-xiang1,LI Jin-hua1,2,3,4,WANG Di2(1.College of Grassland Sciences,Gansu Agricultural University,Lanzhou 730070,China;2.Gansu Key Lab of Crop Improvement and Germplasm Enhancement,Lanzhou 730070,China;3.Key Laboratory of Grassland Ecosystem,Ministry of Education,Lanzhou 730070,China;4.Sino-U.S.Centers for Grazingland Ecosystem Sustainability,Lanzhou 730070,China)  
Rhizosphere soils of potato(Solanum tuberosum) were sampled via a chess-board pattern from fields of potato grown for one year,continuously for 2 years,3 years and 4 years at 4 growing stages(before planting,squaring stage,maturity stage and harvest stage).The soils were isolated on CVP medium via the dilution method for soft rot Erwinia and 42 strains were obtained.During observations of morphology and tests of their physiological and biochemical characteristics,it was found that 4 of the 42 strains shared the same characteristics but differed from the other strains.The 4 strains were GAUP-b410,GAUP-b492,GAUP-b416 and GAUP-d34.The pathogenicity tests indicated that these 4 strains were artificially pathogenetic to cut potato pieces.GAUP-b410 and GAUP-b492 were taken at random for analysis of 16S rDNA sequence,which showed 99% sequence identity with 18 E.carotovora subsp.carotovora strains from Chinese cabbage in China after aligning data in GenBank.Microsoft DNAStar was used to draw the phylogenetic tree of strain GAUP-b410 and GAUP-b492.The tree showed that strain GAUP-b410 was in the same cluster as the 18 Chinese strains,while strain GAUP-b492 was in the same cluster as the 16 of 18 Chinese strains.Therefore,based on the physiological and biochemical characteristics of the 4 strains and the result of 16S rDNA sequence analysis of the 2 strains,the 4 strains were identified as the E.carotovora subsp.carotovora,which were 4 new E.carotovora subsp.carotovora strains.
【Fund】: 甘肃省农业生物技术研究与应用开发项目(GNSW-2006-01);; 现代农业产业技术体系建设专项资金(CARS-10-P18);; 国家科技支撑计划项目(2006BAD21B05)资助
【CateGory Index】: S435.32
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 7 Hits
1 ZHANG Cheng-xia1,2,NAN Zhi-biao2(1.Landscape Architecture Faculty,Southwest Forestry College,Yunnan,Kunming 650224,China;2.Pastoral Agriculture Science and Technology College,Lanzhou University,Gansu,Lanzhou 730020,China);Research progress on effect of grazing on soil microbes of grassland[J];Pratacultural Science;2010-01
2 RONG Li1,LI Xian-wei1,ZHU Tian-hui2,ZHANG Jian1,YUAN Wei-yang1,WANG Qiao1(1.State Key Laboratory of Ecological Forestry Engineering,Sichuan Agricultural University,Ya'an 625014,China;2.State Key Laboratory of Forest Protection,Sichuan Agricultural University,Ya'an 625014,China);Varieties of soil microorganisms decomposing Betula luminifera fine roots and Hemarthria compressa roots[J];Acta Prataculturae Sinica;2009-04
3 ZHAO You-yi1,2,CAI Li-qun1,WANG Jing1,ZHANG Ren-zhi1(1.Resources and Environment Faculty of Gansu Agricultural University,Lanzhou 730070,China;2.Lanzhou City University,Lanzhou 730070,China);Effects of different conservation tillage on amount and distribution of soil ammonifying bacteria,nitrobacteria and nitrogen-fixing bacteria[J];Acta Prataculturae Sinica;2009-04
4 WANG Xu1,2,ZENG Zhao-hai1,ZHU Bo1,HU Yue-gao1,LIN Ye-chun1,CHEN Gong1,LU Jin-sheng2,YUAN Xi-lan2(1.College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China;2.Agricultural Science Institute in Daxing District,Beijing 102600,China);Effects of oat mixed with common vetch on the microorganism populations in rhizosphere soil[J];Acta Prataculturae Sinica;2009-06
5 JIA Yunhe,ZHANG Junhua,CUI Chongshi (College of Horticulture, Northeast Agricultural University, Harbin 150030, China);Research progress on molecular genetics of pathogenicity of the soft rot Erwinia[J];Journal of Northeast Agricultural University;2007-01
6 XU Fei, DAI Xin, CHEN Yue Qin, ZHOU Hui, CAI Jian Hua , QU Liang Hu (School of Life Science,Zhongshan University,Guangzhou,510275)  (South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou,510301);PHYLOGENETIC DIVERSITY OF BACTERIA AND ARCHAEA IN THE NANSHA MARINE SEDIMENTS, AS DETERMINED BY 16S rDNA ANALYSIS[J];Oceanologia Et Limnologia Sinica;2004-01
7 LIU Jin-bo1,2,XU Yan-li1 (1.Northeast Institute of Geography and Agroecology,CAS,Harbin 150081,China;2.Graduate School,CAS,Beijing 100049,China);Current research of soil microbial of successive soybean cropping in China[J];Chinese Journal of Oil Crop Sciences;2008-01
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Jin Junran Huang Yiping Qi Yueqiang (Forest Resources College,BFU) Xu Haifeng (Gong Qing F0rest Farm,Beijing);Bacterial Populations and Properties in Soils under Three Different Stands[J];Journal of Beijing Forestry University;1991-02
2 Zhao Ji(Institute of Natural Resources, Inner Mongolia University, Hohhot 010021);Effect of Stocking Rates on Soil Microbial Number and Biomass in Steppe[J];ACTA AGRESTIA SINICA;1999-03
3 ZHANG Jin-xia, CAO Guang-min, ZHOU Dang-wei, ZHAO Xin-quan (Northwest Plateau Institute of Biology,Chinese Academy of Sciences,Xining 810001,China);The Rate of Carbon Dioxide Release from Alpine Scrubby Meadow Soil Under Different Grazing Intensity[J];Acta Agrestia Sinica;2001-03
4 MA Shuang, YANG Chen-de ,XUE Li,CHEN Xiu-rong (Plant Protection Department of Grassland Science College, Gansu Agricultural University, Lanzhou 730070, China);Seasonal dynamic of soil microorganism in typical grassland under different carrying capacities in Longdong of Gansu[J];Grassland and Turf;2004-02
5 FAN Li-qin~(1),NAN Zhi-biao~(2),SHEN Yu-ying~(2),GAO Chong-yue~(2)(1. College of Prataculture,Gansu Agricultural University,Lanzhou 730070, China 2.College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020, China);Effects of conservation tillage practices on soil microbial biomass carbon in wheat field in the Loess Plateau[J];Grassland and Turf;2005-04
6 Xu Changlin Zhang Pujin(Tianzhu Experimental Station of the Alpine Grassland of Gansu Agricultural University);THE STUDY OF THE COMBINATIONS OF THE MIXTURE ABOUT OATS WITH PEA IN THE COLD PASTORAL AREA[J];Pratacultural Science;1989-05
7 Chang Gengzhu Li Shihang (Lanzhou Institute of Animal Science,Chinese Academy of Agricultural Sciences,Lanzhou 730050);AN EXPERIMENT OF MIXED SOWING AVENA SATIVA AND VICIA SATIVA IN-ZHUONI,GANSU[J];Pratacultural Science;1991-06
8 YAO Tuo,ZHANG De-gang,HU Zi-zhi (Pratacultural College,Gansu Agricultural University,Lanzhou 730070,China);Associative nitrogen-fixing bacteria in the rhizosphere of Avena sativa in an alpine region Ⅰ Isolation and identification[J];Acta Pratacultural Science;2004-02
9 ZHANG Xue-quan~(1,2),HU Ting-xing~1,YE Chong~1,ZHANG Jian~1(1.College of Forestry and Horticulture,Sichuan Agricultural University,the Provincial Key Laboratory of Ecological Engineering of Sichuan Province,Ya'an 625014,China;2.Xichang College,Xichang 615013,China);The net photosynthetic rate and growth of Hemarthria compressa under different trees-grasses shading environments and its economic analysis[J];Acta Prataculturae Sinica;2006-02
10 YAO Tuo~1,LONG Rui-jun~(1,2)(1.Pratacultural College,Gansu Agricultural University,Lanzhou 730070,China;2.Northwest Institute of Plateau Biology,CAS,Xi'ning 810008,China);Dynamics of soil microbial population under disturbance in Tianzhu Alpine grassland[J];Acta Prataculturae Sinica;2006-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved