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《Journal of Northeast Agricultural University》 1999-04
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STUDY OF RAPD ON FOWLS BREEDS

LIU Di (Northeast Agricultural University,Heilongjiang Harbin 150030,PRC)  
Random amplified polymorphic DNA(RAPD)technique was applied to assess the genetic variations in six breeds with 12 random primer given 223 reproducible amplified fragements.The polymorphic fragement was 188 bands(84 3%),that reflected genetic variations in breeds and among breeds.Amplification with 12 primer checked out 16,21,22,22,17,17,20,22,12,18,23,18 and 16 bands for sixty individuals. The scope of genetic similarity variations was 0 798 1~ 0 868 5 in six fowls breeds 0 599 3 ~ 0 781 3 among six fowl breeds.The variations was the biggest in Abor Acres.The genetic distance was 0 4007 between Abor Acres and ISA Brow. The cluster analysis indicated a distant relationship between Abor Acres and five egg line fowl breeds.As the same time,amplification with 12 primer given 7 special bands and 3 bands was interrelated with sex.
【Fund】: 黑龙江省自然科学基金! C950 9
【CateGory Index】: S831;S83
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