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《Journal of Northeast Forestry University》 2014-01
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Crossbreeding Barriers of Interspecific Hybridization between Iris halophila Pall and I. pseudocorus L.

Yang Zhan-hui;Shi Yanyan;Gao Yike;Zhan Qixiang;Beijing Forestry University;  
The experiment was conducted to study the crossbreeding barriers between two Iris species,I. halophila Pall and I.pseudocorus L.,and determine the pollen viability,pollen tube behaviors after common pollination,embryology observation and embryo rescue. Post-zygotic barrier is the main reason for the cross incompatibility between I. halophila and I. pseudacorus,and it expresses embryo abortion in early zygophase. No capsules are obtained from reciprocal crosses,and there are pro-zygotic barriers in the two combinations. The stylar canal after 48 hours pollination in I. halophila ×I. pseudacorus is blocked by a large number of embolism,while after two hours pollination in I. pseudacorus×I. halophila some corpus callosum appeares on stigma. However,there is still a small amount of pollen tube at ovules of both combinations ultimately.The failures of embryo and endosperm in I. halophila×I. pseudacorus and I. pseudacorus×I. halophila,respectively,occur at 9-15 days and 15- 21 days after pollination. Six days after pollination is the appropriate time for embryo rescue of I.pseudocorus×I. halophila,and there are inflated ovules in medium of MS+2.0 mg·L-16-BA.
【Fund】: 国家林业局“948”引进项目(2013-4-46)
【CateGory Index】: S682.19;S682.32
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