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Ray Induced Changes in BEP2D Cell Protein Expression

Xue Zhenwei Zhou Xiaolin Cui Meiping(China Institute for Radiation Protection,Taiyuan,030006)  
The samples of the BEP2D cell radiated with 0、1.5 and 3.0 Gy γ rays were cultivated and collected,and the total protein were separated with the two-dimensional gel electrophoresis.The peptide mass fingerprint were obtained using matrix-assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF-MS)for differentially expressed proteins.10 peptide mass fingerprinting are reveal in the mass spectra identification,8 of them are approved in the database,and 1 are duplicated;2 are null. There are 7 points identified in the initial testsby Mascot software in the NCBInr database,such as Cyclophilin A,Myosin light chain 2,Cytokeratin 9,α-enolase,Triosephosphate isomerase 1,Makorin ring finger protein 1,c-myc promoter-binding protein 1.these 7 kinds of proteins probablely are the special proteins related to the lung cancer induced by irradiation,which might be worth of further study of the functions of proteins.
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