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《Academic Journal of Second Military Medical University》 1984-03
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METHOD FOR ULTRASTRUCTURAL LOCALIZATION OF N ACETYLCHOLINE RECEPTOR

Chen Zhi, Zheng Zun, Jiang Luli, Yang YushanDepartment of Biochemistry and Central Laboratory  
A method by using α-BT-HRP conjugate to localize N-acetylcholinc receptor of neuromuscular junction was described.Thin strips of fresh muscle were incubated with α-BT-HRP conjugate at a concentration of 1×10-7 M in Tyroid's solution and then reacted with Karnovsky's DAB medium.There were obviously brownish red positive deposits to constitute various vesicular forms seen under a light microscope. Under an electron microscope we can see positive deposits that were localized both on the presynaptic and postsynaptic membra-nes. The binding activity and reliability of a-BT-HRP were discussed.
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【References】
Chinese Journal Full-text Database 2 Hits
1 Yang Yushan,et al(Department of Biochemistry);A STUDY OF SOME PROPERTIES OF α-BT-HRP CONJUGATES AND THE PERIODATE METHOD OF PREPARATION[J];Academic Journal of Second Military Medical University;1985-03
2 Hu Huimin, et al (Department of Biochemistry);PRELIMINARY STUDY ON THE PREPARATION OF α-BT-HRP CONJUGATES BY PERIODATE AND GLUTARALDEHYDE METHODS[J];Academic Journal of Second Military Medical University;1987-02
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 Mao Qingwu,et alDepartment of Medical Protection, et al;STUDIES OF ISOLATION, PURIFICATION AND IODINATION OF α-BUNGAROTOXIN[J];Academic Journal of Second Military Medical University;1981-04
2 Yang Yushan,et al(Department of Biochemistry);A STUDY OF SOME PROPERTIES OF α-BT-HRP CONJUGATES AND THE PERIODATE METHOD OF PREPARATION[J];Academic Journal of Second Military Medical University;1985-03
3 Hu Huimin, et al (Department of Biochemistry);PRELIMINARY STUDY ON THE PREPARATION OF α-BT-HRP CONJUGATES BY PERIODATE AND GLUTARALDEHYDE METHODS[J];Academic Journal of Second Military Medical University;1987-02
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 Hu Huimin, et al (Department of Biochemistry);PRELIMINARY STUDY ON THE PREPARATION OF α-BT-HRP CONJUGATES BY PERIODATE AND GLUTARALDEHYDE METHODS[J];Academic Journal of Second Military Medical University;1987-02
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