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《Academic Journal of Second Military Medical University》 1990-01
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In Situ Hybridization and RNA Dot Blot Analysis of Alpha-fetoprotein Messenger RNA in Human Hepatocellular Carcinoma and Its Host Liver

Yang Linseng, et al (Department of Pathoanatomy)  
The expression of the alpha-fetoprotein in 12 cases of human hepatocellular carcinoma and its host liver by in situ hybridization analysis of AFP mRNA in cryostat and paraffin - embeded tissue sections was studied. Total RNA was extracted in 3 cases of cancer tissues and its host liver tissues for RNA spot blot Both the tumor cells and the surrounding host liver cells showed positive hybridization with a biotinylated AFP cDNA probe. AFP mRNA existed in almost all tumor cells, but the distribution pattern of AFP mRNA in the host liver varied. The positive cells were dispersed in the tissue or formed foci or appeared to be a small piece of positive area. Our results showed that not only the tumor cells can synthesize AFP, but also the surrounding liver cells have the capacity to produce AFP.
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【Co-citations】
Chinese Journal Full-text Database 3 Hits
1 HUANG Pei-sheng CHEN Yun-ying WANG Yong (Department of pathology, Fujian Medical College, Fuzhou);a-FETOPROTEIN IN HEPATOCELLULAR CARCINOMA TISSUE[J];Chinese Journal of Cancer;1989-03
2 Tao Wenzhao, et al Department of pathological Anatomy;PATHOLOGICAL OBSERVATIONS ON THE ENZYME-LABELING IMMUNO-FETOPROTEIN IN HUMAN NONCIRRHOTIC LIVER CANCER AND PERI-CANCER TISSUE[J];Academic Journal of Second Military Medical University;1985-01
3 Li Lie Lany zhiren Department of Pathology;The Developing Observation of Liver Carcinogenesis of The Rats[J];Academic Journal of Guangzhou Medical College;1988-04
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 Dai Yimin, et al (Department of Pathological Anatomy);DOUBLE IMMUNOLOCALIZATION OF AFP, HBsAg, IgG AND ALBUMIN IN HUMAN LIVER CANCER AND ITS SURROUNDING LIVER TISSUE[J];Academic Journal of Second Military Medical University;1989-04
2 Zheng Jie; WU Zhongbi; Ruan Youbing; et al(Tongji Medical Uniuersity,Wuhan 430030);A study of expression of ras p21and AFP in experimental precancerous lesion of liver in rats[J];CHINESE JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOG;1995-02
3 Zhang Xinli; Shi Jingquan;Wang Xiaodong; et al(Dept of Pathol, Third Military Medical University,Chongqing, 630038);Study on morphometric analysis and proliferative activities of AFP-positive cells in human hepatocellular carcinoma and their adjacent liver tissues[J];CHINESE JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOG;1996-01
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