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《Academic Journal of Second Military Medical University》 1990-01
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HPLC Determination and Phaimacokinetic Investigation of Propafenone in Heahhy Volunteers

Liu Gaolin, et al (Department of Clinical Pharmacology, Changhai Hospital)  
A RP-HPLC method was described for the determination of plasma propafenone. The column was 25 cm×4. 0 mm ID stainless steel packed with ODS (5 μm). The mobile phase consisted of CH3OH: HAC-NaAC: H2O (70: 15: 15, v/v), containing 0. 0584 mol/L diethylamine as a modifier. The flow rate was 1. 0 ml/min. Tetracaine was used as a internal standard. Chromatography was performed at 250 run with variable wavelength ultraviolet detector. The propafenone was first extracted from alkalized plasma with 2.0 ml of 2% (v/v) isoamyl alcohol in n-heptane, and then reextracted with 0. 3 mol/L H3PO4, 100μl. Linear calibration curve for propafenone was measured over the range of 50-1600 ng/ml, and correlation coefficient was 0. 9993. The recovery rate of the method was 88.82±6.39% (n =5), and the coefficient of variation was less than 10% (n = 10). The lowest determined level was 40 ng/ml.The pharmacokinetic parameters were measured by this method in 5 healthy volunteers after a single oral dose propafenone 300 mg. The mean pharmacokinetic parameters were as follows: t1/2 (Ka=0. 40±0. 15 h, t1/2 (K) =2. 81±0. 52 h, Tmax= 1. 88±0. 47 h, Cmax= 631. 67±453. 13 ng/ml, and AUC= 3843.06±3032. 80ng·h-1·ml-1..
【Fund】: 第二军医大学青年科研基金
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【References】
Chinese Journal Full-text Database 3 Hits
1 Liu Gaolin, et al (Department of Clinical Pharmacology, Changhai Hospital);Double Drug Interaction between Propafenone and Digoxin[J];Academic Journal of Second Military Medical University;1990-04
2 Chen Bing,Cai Weimin,Wan Wei Departmentof Clinical Pharmacology,Jinling H ospital(Nanjing,2 1 0 0 0 2 );Determination of propafenone enantiomeric concentrations in human plasma by stereoselective high performance liquid chromatography[J];BULLETIN OF JINLING HOSPITAL;1997-04
3 Ren Ke-qin,Han Bo-qun,Zhu Jun(Central laboratory Cardiac medicine, Shanghai second people’s hospital,200011 );ABSTRACTS OF ORIGINAL ARTICLES Determination of propafenone in serum for elder patients by HPLC[J];CHINESE JOURNAL OF HOSPITAL PHARMACY;1994-03
【Co-references】
Chinese Journal Full-text Database 6 Hits
1 CHEN Qiu-Chao;ZHU Jun-Ren;JIANG Wen-De;LI Zhi-Shan;SHEN Ming-Hua;CHEN Wei-Li;YANG Jin-Ming Zhong-Shan Hospital; Department of Pharmacology, Shanghai First Medical College, Shanghai;HPLC ANALYSIS OF PROPAFENONE CONCENTRATION IN HUMAN PLASMA FOR DEFERMING ITS PHARMACOKINETIC STUDY[J];The Chinese Journal of Clinical Pharmacology;1985-01
2 LI Jun-Yi;TAO Ping Department of Clinical Pharmacology, Fu Wai Hospital, Chinese Academy of Medical Sciences, Beijing;RELATIONSHIP BETWEEN PBOPAFENONE DOSE AND SERUM CONCENTRATION[J];The Chinese Journal of Clinical Pharmacology;1989-04
3 LI Jun-Yi;TAO Ping Department of Clinical Pharmacology, FU Wai Hospital Chinese Academy of Medical Sciences Beijing, 100037;PROPAFENONE PHARMACOKINETICS AFTER SINGLE ORAL ADMINISTRATION[J];The Chinese Journal of Clinical Pharmacology;1990-02
4 WL Zhang;YN Tang Department of Pharmacy, Xinhua Hospital of Shanghai Second Medical University, Shanghai 200092;SIMULTANEOUS DETERMINATION OF PROPAFENONE AND ITS ACTIVE METABOLITE IN SERUM BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY[J];Acta Pharmaceutica Sinica;1992-07
5 Zhang Xian-zhou,Cai Hong-sen,Luo Shun-de,et al(The People’s Hospital of Hubei Province 430060);Study on pharmacokinetics of propafenone in arrhythmia patients[J];CHINESE JOURNAL OF HOSPITAL PHARMACY;1994-01
6 Ren Ke-qin,Han Bo-qun,Zhu Jun(Central laboratory Cardiac medicine, Shanghai second people’s hospital,200011 );ABSTRACTS OF ORIGINAL ARTICLES Determination of propafenone in serum for elder patients by HPLC[J];CHINESE JOURNAL OF HOSPITAL PHARMACY;1994-03
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 ZHANG Jin an 1,CHEN Chao jun 2,XIANG Yan ying 3,ZHANG Yi 4(1. Department of Pharmacy, Hospital of the People's Armed Police Forces of Hubei Province Corps, Wuhan 430070, China ; 2. Wuhan Hualong Bio Chemical Pharmaceutical Co. Ltd., Wuhan 430040, China ; 3. Department of Pharmacy No.161 Hospital of the PLA, Wuhan 430030, China ;4. Department of Physiology, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China );A Study of Propafenone Enantiomers in the Human Plasma with High Performance Liquid Chromatography[J];Herald of Medicine;2003-08
2 HU Xiao-yu,YAO Tong-wei (College of Pharmaceutical Sciences,Zhejiang University,Hangzhou 310031,China);Interaction between ipriflavone and other drugs in metabolism in vitro[J];Journal of Zhejiang University(Medicine Sciences);2004-01
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