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《Academic Journal of Second Military Medical University》 1991-06
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Ultrastructural Study of Human Epidermal Keratinocytes Cultured in Low Calcium Medium

Dai Fangping, Liu Junlong, Chen Yulin, Wang Wenzheng (Department of Bum, Changhai Hospital, Shanghai, 200433)  
A low calcium medium developed for epidermal keratinocytes were prepared according to the MCDB 153 modified formula and used in human epidermal keratinocyte culture compared with DMEM culture system. The observation by contrast microscopy and electron microscopy showed that in the low calcium medium keratinocytes grew as a monolayer of high proliferation and had many characteristics of basal cells, with a more rounded shape and large intercellular spaces. Increasing the calcium ion concentration in the medium or changing the other culture conditions the cells in these cultures could be induced stratification and terminal differentiation. The results suggest that the growth, proliferation and differentiation of cultured human epidermal keratinocytes can be controlled and regulated someway.
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Wang Wenzheng, et al (Department of Burn, Changhai Hospital);CULTIVATION OF HUMAN EPIDERMAL CELLS ON FIBRONECTIN COATED SUBSTRATE AND I'TS CLINICAL APPLICATION[J];Academic Journal of Second Military Medical University;1988-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Wang Wenzheng, et al. (Burn Unit, First Teaching Hospital);STUDIES ON THE CULTIVATION OF EPIDERMAL CELLS AND TRANSPLANTATlON: I.GROWTH OF CULTURED HUMAN EPIDERMAL KERATINGCYTES IN THREE CULTURE MODELS.[J];Academic Journal of Second Military Medical University;1985-06
2 Wang Wenzheng, et al (Department of Burn, Changhai Hospital);CULTIVATION OF HUMAN EPIDERMAL CELLS ON FIBRONECTIN COATED SUBSTRATE AND I'TS CLINICAL APPLICATION[J];Academic Journal of Second Military Medical University;1988-02
3 Dong Zhaoyang, Chen Yulin, Dai Fangping, Huan Jinning, Zhang Suzhen, Ying Kang, Lü Luo, Fan Qingyuan, Fang Zhiyang (Department of Burn, Changhai Hospital, Second Military Medical University, Shanghai, 200433);The expression level of the transfected hEGF gene to cultured human epidermal cells[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1998-S1
4 Chen Bi(Department of Plastic Surgery and Burns, First Affiliated Hospital);Culture and Grafting of Human Epidermal Cells[J];Journal of The Fourth Military Medical University;1987-03
5 Cui Zhengjun 1, Chen Bi 1, Tang Chaowu 1, Shi Xinyou 2, Shen Lizhong 3, Jiang Duyin 1 1Department of Burn, Xijing Hospital, Fourth Military Medical University, Xi'an 710033 2Research Center of Laboratory Animal, 3Department of Imm;Preliminary observation of allo epidermis and allo composite skin grafting[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1997-02
6 SU Ying Jun, CHEN Bi, TANG Chao Wu, HU Da Hai, JIANG Du Yin, JIA Chi Yu Department of Burn Surgery,Center of Plastic Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an 710033, Shaanxi Province,China;In vitro transformation of human keratinocytes by simian virus 40[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1999-05
7 SU Ying Jun, CHEN Bi, JIA Chi Yu,TANG Chao Wu Department of Burn Surgery, Centre of Plastic Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an 710033, Shaanxi Province, China;Biological characteristics of long term in vitro culture of SV40 transformed human keratinocyte line[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1999-05
8 HU Da Hai, CHEN Bi, TANG Chao Wu, XU Ming Da, JIA Chi Yu Department of Burn Surgery, Center of Plastic Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an 710033, Shaanxi Province, China;Biological effects of pcDNA 3 hbFGF modified human keratinocytes on the proliferation of human dermal fibroblastes[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1999-05
9 Zhao Xiongfei,Zhang Shijun.Hu Jianian.Lu Shuzhen,Wang Xu and Li Ngao.Burn Institute,Third Military Medical College,Chongqing;CULTURED HUMAN EPIDERMAL CELL SHEETS SERVE AS ALLOGENIC COVERING ON BURN WOUND[J];Medical Journal of Chinese People's Liberation Army;1989-02
10 SU Bo,SUN Jia ling,WANG Wen zheng,et al. Burn Institute,Changhai Hospital, 2nd Military Medical University,Shanghai 200433;Composite skin Reconstituted by Cultured Autologous Epidermic Cells and Allogenic Dermis:An Animal Experiment and Clinical Application[J];CHINESE JOURNAL OF TRAUMATOLOGY;1995-03
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Wang Wenzheng, et al. (Burn Unit, First Teaching Hospital);STUDIES ON THE CULTIVATION OF EPIDERMAL CELLS AND TRANSPLANTATlON: I.GROWTH OF CULTURED HUMAN EPIDERMAL KERATINGCYTES IN THREE CULTURE MODELS.[J];Academic Journal of Second Military Medical University;1985-06
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