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《Academic Journal of Second Military Medical University》 2012-01
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Effect of tubulin tyrosine ligase like 12 and nitrotyrosine on growth of prostate cancer cells

LI Ya-dong1,ZHANG Jin-song1,YANG Kai1,ZHANG Fu-jun1,CHEN Rui1,HONG Su-ling2 1.Department of Oral and Maxillofacial Surgery,The First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China 2.Department of Otolaryngology Head and Neck Surgery,The First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China  
Objective To study the effect of tubulin tyrosine ligase like 12(TTLL12) and nitrotyrosine on the growth of prostate cancer cells.Methods TTLL12 and α-tubulin nitrotyrosination(N-tubulin) were detected by Western blotting analysis in normal prostate epithelial cell lines PWR1E and RWPE1,and prostate cancer cell line DU145;the proliferation of cells was observed by MTT assay.Results The expression of N-tubulin was significantly lower in DU145 cells than those in PWR1E and RWPE1 cells after treatment with nitrotyrosine(P0.05).The proliferation of PWR1E and RWPE1 cells treated with nitrotyrosine(cultured with 800 μmol/L nitrotyrosine) were significantly inhibited compared with the control(cultured with 800 μmol/L hydrochloric acid,P0.05),while there was no visible change in the proliferation of DU145 cells.N-tubulin level was significantly higher in the experimental group(transfected with siTTLL-12) than the control group(transfected with siControl) after effective silence of TTLL12(P0.05);the proliferation of DU145 cells treated with nitrotyrosine in the experimental group was significantly inhibited(P0.05),while there was no visible change in cell proliferation in the control group (P0.05).Conclusion TTLL12 allows an escape of prostate cancer cells from the treatment of nitrotyrosine,leading to the escape of prostate cancer cells from the body's surveillance system and acquirement of their abnormal proliferation.
【Fund】: 重庆市卫生局医学科研计划项目(2011-2-013)~~
【CateGory Index】: R737.25
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