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《Academic Journal of Second Military Medical University》 2012-01
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Mechanism on hepatitis B virus X gene-induced hepatic steatosis

ZHANG Qin 1,PENG Jun2,SHEN Wei1 1.Department of Gastroenterology,The Second Affiliated Hospital,Chongqing Medical University,Chongqing 400010,China 2.Department of Gynaecology and Obstetrics,First People's Hospital of Liangshan Prefecture,Xichang 615000,Sichuan,China  
Objective To investigate the effect of liver X receptor α(LXRα) gene silencing on lipid metabolism-related genes in HepG2.2.15 cells.Methods HepG2.2.15 cells were divided into blank control group(without transfection),negative control group(transfected with HK plasmid),and shLXRα group(transfected with shLXRα plasmid).The shLXRα plasmids carrying LXRα gene were constructed and were used to transfect HepG2.2.15 cells using PolyJetTM reagent.Green fluorescent protein and LXRα protein expression were examined by fluorescence microscope and Western blotting analysis 24-96 h after transfection,so as to identify the best interference time.Then cells were treated with agonist T0901317 for 24 h or 48 h;and the content of triglyceride(TG) was observed to detect the degree of steatosis by biochemical assay.The expression of sterol regulatory element binding protein-1c(SREBP-1c) mRNA was detected by RT-PCR and the expression of hepatitis B virus X(HBx) protein and fatty acid synthase(FAS) protein was tested by Western blotting analysis.Results The shLXRα plasmid was constructed and transfected into HepG2.2.15 cells successfully.Compared with blank and negative control groups,LXRα protein was markedly decreased in the shLXRα group,with the lowest level found at 48-72 h after transfection(P0.01).After cells were stimulated with T0901317,HBx and FAS protein expression,the content of TG,and SREBP-1c mRNA expression gradually increased with the prolongation of stimulation period,and there was no significant difference in HBx expression at the same time point between different groups.FAS protein,TG contents,and SREBP-1c mRNA in shLXRα group were significantly lower than those in the other two groups(P0.01).Conclusion HBx can regulate lipid metabolism through LXRα/SREBP-1c/FAS pathway.
【Fund】: 国家自然科学基金(30871160)~~
【CateGory Index】: R575.5
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Zhang Qin1,Peng Jun2,Shen Wei1(1Department of Gastroenterology,Second Affiliated Hospital,Chongqing Medical University,Chongqing,400010;2Department of Obstetrics and Gynecology,First People's Hospital of Liangshan Prefecture,Xichang,Sichuan Province,615000,China);Preliminary study of the effect and mechanism of hepatitis B virus X gene in hepatic steatosis[J];Journal of Third Military Medical University;2011-23
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 Chen Juan,Shen Wei(Department of Gastroenterology,Second Affiliated Hospital,Chongqing Medical University,Chongqing,400010,China);Effect of hepatitis B virus X protein on sodium oleate-induced lipid accumulation in HepG2 cells[J];Journal of Third Military Medical University;2012-03
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Guo Qishuai1,Huang Xi2,Zhang Jun1,Li Shaolin1(1Department of Radiation Medicine,Chongqing Medical University,Chongqing,400016;2Department of Oncology,Hechuan Peoples’Hospital,Chongqing,401520,China);Construction of short hairpin RNA eukaryotic expression vectors targeting peroxiredoxinⅠ and identification of their biological functions[J];Acta Academiae Medicinae Militaris Tertiae;2011-02
2 ZHENG Li-li,SHEN Wei,XIONG Ling(Department of Gastroenterology,The Second Affiliated Hospital,Chongqing University of Medical Sciences,Chongqing 400010,China);Relationship between Expression of Hepatitis B Virus X Protein and Hepatic Steatosis and Relevant Mechanism[J];Chinese Journal of Biologicals;2010-09
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