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《Academic Journal of Second Military Medical University》 2012-02
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ERK1/2 pathway-mediated norepinephrine regulates function of endothelial progenitor cells

JIANG Qi-jun,WU Jian-xiang,LIU Xing,LIANG Chun,REN Yu-sheng,WU Zong-guiDepartment of Cardiology,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China  
Objective To analyze the effects of norepinephrine(NE) on the proliferation and migration of endothelial progenitor cells(EPCs) and the related mechanism.Methods NE,adrenoceptor antagonist,and MAPK signal pathway blocker of various concentrations were used to treat peripheral EPCs derived from healthy adults.The proliferation potential,migration capacity and activation of ERK1/2 were assessed after different treatments.Results NE increased the proliferation potential of EPCs in a dose-dependent manner.The number of EPCs increased by(48.3±23.3)%,(70.5±35.6)%,(82.4±14.9)% and(100.3±48.1)% after treatment with NE at 0.01 μmol/L,0.1 μmol/L,1 μmol/L and 10 μmol/L,respectively.Addition of alpha adrenoceptor antagonist phentolamine,selective beta 2 adrenoceptor antagonist I127,JNK blocker SP600125 and ERK1/2 blocker A6355 could block the above effects of NE,and beta 1 adrenoceptor antagonist metoprolol and p38 blocker PD169318 failed to block the effects of NE.NE at 10 μmol/L significantly promoted the migration of EPCs(P0.05).These effects could be blocked by addition of phentolamine(10 μmol/L) and I127(10 μmol/L),but not by addition of metoprolol.NE(0.1,1 and 10 μmol/L) activated ERK1/2 pathway in a dose-dependent manner,which could also be blocked by phentolamine and I127,but not by metoprolol.Conclusion NE can increase the proliferation potential and migration capacity of EPCs via activating ERK1/2 pathway with alpha and beta 2 adrenoceptor.
【Fund】: 国家重点基础研究发展计划(“973”计划)课题(2005CB523309)~~
【CateGory Index】: R329.2
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 GU Ping~(1,2*) WANG Yan-yong~(1,2) MA Qin-ying~1 WEN Ya~1 CUI Dong-sheng~1 LIU Li WANG Ming-wei~(1,2) ZHANG Zhenqing~(1,2) 1.Brain Aging and Cognitive Neuroscience Laboratory of Hebei Province,Shijiazhuang 050031,Chin; 2.Department of Neurology,the First Affiliated Hospital of Hebei Medical University,Shijiazhuang 050031;Influence of ERK1/2 signal-transduction pathway on differentiation of mesencephalic neural stem cells induced by bone marrow stromal cell-conditioned medium[J];Academic Journal of Second Military Medical University;2007-06
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 Qin-Ying Ma1,2,Ya Wen2,Ping Gu1,2,Yan-Yong Wang1,2,Ming-Wei Wang1,2,Dong-Sheng Cui2,Li Liu2(1Department of Neurology,First Hospital Affiliated Hebei Medical University,Shijiazhuang 050031,China;2Hebei key Laboratory for Brain Ageing and Cognitive Neuroscience,Shijiazhuang 050031,China);The Signal Transduction Pathway of NSCs' Differentiation Induced by BMSCs Conditioned Medium[J];Chinese Journal of Cell Biology;2009-03
2 Gu Ping1,2,Zhang Zhong-xia1,Wang Yan-yong1,2,Cui Dong-sheng1,Geng Yuan1,Wang Ming-wei1,2 1Hebei Provincial Laboratory of Brain Aging and Cognitive Neuroscience,Shijiazhuang 050031,Hebei Province,China;2Department of Neurology,First Hospital,Hebei Medical University,Shijiazhuang 050031,Hebei Province,China;Effects of combined transplantation of bone marrow stromal cell conditioned medium and neural stem cells on the behavior and cognition of Parkinson's disease rats:Whether the outcomes of their combination are better than neural stem cell transplantation al[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2010-19
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 GU Ping 1,2 , LOU Shu Jie 1* , WANG Ming Wei 2, WANG Yan Yong 2, BAO Xuan 1, HE Cheng 1, LU Chang Lin 1(1. Department of Neurobiology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433,China; 2. Department of Neurology, the First Hospital, Medical University of Hebei, Shijiazhuang 050031);Effects of bone marrow stromal cell-conditioned medium on differentiation of mesencephalic neural stem cells into neurons[J];Academic Journal of Second Military Medical University;2003-03
2 LOU Shu-Jie, GU Ping, HE Cheng, WANG Ming-Wei, LU Chang-Lin (1 Department of Neurobiology, Second Military Medical University, Shanghai 200433 2 Department of Neurology, First Affiliated Hospital of Hebei Medical University);Inducing action of bone marrow stromal cells on neuronal differentiation of mesencephalic neural stem cells[J];Chinese Journal of Anatomy;2002-06
3 LOU Shu jie 1 , GU Ping 1,2 ,LI Yi 1 ,CHEN Fei 1 ,ZHANG Ming hui 3 ,HE Cheng 1 ,WANG Ming wei,LU Chang lin 1 ( 1.Department of Neurobiology, Second Military Medical University, Shanghai 200433 ;2.Department of Neurology,;Effect of bone marrow stromal cells on the differentiation of neural stem cells into neurons[J];Chinese Journal of Neuroscience;2002-02
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