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《Academic Journal of Second Military Medical University》 2012-02
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Establishment of patch clamp and laser scanning confocal microscope synchronous real-time control system and its application in study of membranaceous calcium channel

LIU Hong1,WANG Ye1,TANG Ying2,SHEN Ya-feng2,YANG Yong-ji2,LEI Chang-hai1 1.The Network Information Center,Second Military Medical University,Shanghai 200433,China 2.Department of Biophysics,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433,China  
Objective To establish a patch clamp and laser scanning confocal microscope synchronous real-time control system,and apply it in study of membranaceous calcium channel,so as to verify its effects.Methods By adding the patch clamp on the laser scanning confocal microscope,we established a patch clamp and laser scanning confocal microscope synchronous real-time control system using computer autocontrol technique.The system was used to observe the male rat membranaceous calcium channel in vitro,and the observation results were analyzed.Results We successfully established the patch clamp and laser scanning confocal microscope synchronous real-time control system.When the myocardial cells were stimulated,the system could observe the myocardial calcium spark by laser scanning confocal microscope and at the same time record calcium channel current signal by patch-clamp.Quantitative analysis showed that the time intervals between 2 adjacent calcium sparks were(10.055±0.021),(10.079±0.021) and(10.087±0.021) s,which met the stimulus interval for patch-clamp set(10 s).A single calcium spark had a diameter within 2 μm,and it experienced an average period of about 30 ms.It needed an average of 10 ms from the appearance of the spark to its peak concentrations,and it needed an average of 20 ms from its peak concentration to disappearance,which was consistent with the calcium spark theory.Conclusion A patch clamp and laser scanning confocal microscope synchronous real-time control system has been successfully established.The system is capable of performing whole-cell observation recording and determining the membranaceous calcium channel currents and its closing process by patch clamp; at the same time it can synchronously obtain the micro images of the calcium spark with laser scanning confocal microscope,locating changes of calcium ions and helping to understand the internal mechanism of the membrane calcium channels.
【Fund】: 国家自然科学基金(39970359 30170253)~~
【CateGory Index】: R331
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 BI Yu-hua1, CHEN Wen-li1,2, XING Da2 (1. College of Life Science,Guangdong Key Lab of Biotechnology for Plant Development, South China Normal University,Guangzhou 510631, China;2. MOE Key Laboratory of Laser Life Science and Institute of Laser,South China Normal University,Guangzhou 510631, China);APPLICATION OF DELAYED FLUORESCENCE ANALYSIS AND LASER SCANNING CONFOCAL MICROSCOPE IN DETECTING CHLOROPHYLL CONTENT OF Gerbera hybrida EPIDERMAL CELL DURING ITS DEVELOPMENT[J];Acta Biophysica Sinica;2007-05
2 ZHANG Wei-wei1,2,ZHOU Chen1,QI Cui1,BAO Chao-fei1,JING Jun-zhan1,ZHANG Li-yao1,HAN Su-e1 (1.The College of Life Sciences,HeBei University,Baoding 071002,China;2.School of Basic Medical Science,HeBei University,Baoding 071000,China);POTASSIUM CURRENTS IN NEURONS OF RATS WERE REDUCED BY IL-1β IN TIME DEPENDENT MANNER[J];Acta Biophysica Sinica;2009-06
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 QI Cui1,ZHANG Wei-Wei2,WANG Fei1,BAO Chao-Fei1,WANG Xin-Wei1,LI Xiao-Nan1,YU Xiao-Qing1,ZHOU Chen1,*(1.College of Life Sciences,Hebei University,Baoding 071002,China;2.School of Basic Medical Science,Hebei University,Baoding 071000,China;Acute effects of IL-1β on sodium current in cortical neurons of rats[J];Zoological Research;2011-03
2 Zhou Lin,Wang Yan,Lü Chunyan,Peng Zhenhua(Key Laboratory of Tree Breeding and Cultivation(Research Institute of Forestry,Chinese Academy of Forestry),State Forestry Administration,Beijing 100091,P.R.China);Identification of Components of Flower Pigments in Petals of Paeonia lutea Wild Population in Yunnan[J];Journal of Northeast Forestry University;2011-08
3 CHEN Hai-xiaa,b, LIU Ming-yuea, Lü Chang-pingb* (a.College of Horticulture and Landscape; b. Ornamental Horticulture Research Institution, HNAU, Changsha 410128, China);Effect of pigment distribution on the color expression of Gerbera hybrida petal[J];Journal of Hunan Agricultural University(Natural Sciences);2010-02
4 QI Cui1,ZHANG Wei-Wei2,LI Xiao-Nan1,ZHOU Chen1,1The College of Life Sciences,Hebei University,Baoding 071002,China;2School of Basic Medical Science,Hebei University,Baoding 071000,China;Interleukin-1β inhibits the amplitudes of voltage-gated Na~+ currents and action potential in cultured cortical neurons of rat[J];Acta Physiologica Sinica;2011-02
5 YANG Liu1,2,DONG Junjian1,2,CHEN Wenli1,2 1.College of Life Science,Guangdong Key Lab of Biotechnology for Plant Development, South China Normal University,Guangzhou 510631,China; 2.MOE Key Laboratory of Laser Life Science,South China Normal University,Guangzhou 510631,China;Autophagy and Declined Photosynthesis in Arabidopsis during Pathogenic Pst DC3000 Infection[J];Acta Biophysica Sinica;2011-06
China Proceedings of conference Full-text Database 1 Hits
1 ZHOU Lin WANG Yan LV Chun-yan PENG Zhen-hua (Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091);Primary Analysis and Identification of Flower Pigments Components in the Petals of Paeonia lutea Wild Population in Yunnan[A];[C];2011
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 Guo Zhouyi,Zhu Yanbin,Ma Junfu,L i Shaoshan Laser Lif e Science Institute of South China Normal University,Guangzhou 51 0 63 1;THE SPECTRA DISTRIBUTION PROPERTIESOF ULTRAWEAK PHOTON EMISSION FROM BIOLOGICAL SYSTEM AND BIOPHOTON COHERENCE[J];ACTA PHOTONICA SINICA;2000-10
2 JIN Hong-Wei, ZHANG Wei, QU Lin-Tao, WANG Xiao-Liang Institute of Materia Medica, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100050;Electrophysiological correspondence between Kv4. 2 current and transient outward potassium current in the cultured rat hippocampal neuron[J];Acta Physiological Sinica;2003-06
3 PAN Jianping 1, LIU Lieju 1, 2, YANG Fei 1, CAO Xuehong , FU Hui 1, MING Zhangxin 1 1 Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China 2 Departments of Anesthesiology, Duke University Medical Center, Durham, NC 27710, USA;Effect of Interleukin-1β on I_A and I_K Currents in Cultured Murine Trigeminal Ganglion Neurons[J];华中科技大学学报(医学英德文版);2007-02
4 HUANG Yong~1,ZHOU Ji-Heng~(1,*),YANG Hong-Qi~1,LIU Jian-Li~3,WANG Yong~4,ZHOU Yi-He~3,HUANG Yan~1 and HE Xiao-Li~2(()~1 Biology Science and Technology Department,Tobacco Science and Engineer Key Laboratory;()~ 2Horticulture and gardens college,Huanan Agricultural University,Changsha 410128;Hunan;()~3 China Tobacco Purchase and Sale Company,Beijing 100055;()~ 4 Changde Quanyan Company Technology Center,Changde 415000,Hunan,China);Cytological Observation of Leaf and Chloroplast during the Flue-Cured Tobacco Maturation[J];Acta Agronomica Sinica;2006-11
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