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《Academic Journal of Second Military Medical University》 2012-02
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Decreased miR-302e expression in monocytes of patients with primary biliary cirrhosis and its significance

LIU Xiao1,HU Zhi-de2,3,DENG An-mei2,ZHONG Ren-qian1 1.Department of Laboratory Diagnosis,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China 2.Department of Laboratory Diagnosis,Changhai Hospital,Second Military Medical University,Shanghai 200433,China 3.Department of Laboratory Medicine,General Hospital of PLA Ji'nan Military Area Command,Ji'nan 250031,Shandong,China  
Objective To investigate the role of miR-302e in the pathogenesis of primary biliary cirrhosis(PBC).Methods The relative expression levels of miR-302e in T cells(CD3+),B cells(CD19+) and monocyte(CD14+) of 10 healthy controls and 12 PBC patients were detected by RT-PCR.The THP-1 cells transfected with miR-302e mimics or inhibitors and the monocytes from PBC patients and healthy controls were treated with 100 ng/ml bacteria lipopolysaccharide(LPS).Twenty-four hours later,the concentrations of IL-6 and TNF-α in the culture medium were measured by ELISA.Results The monocytes of PBC patients had a significantly lower miR-302e expression compared to those of healthy controls(P0.01).miR-302e inhibitors significantly enhanced the production of IL-6 and TNF-α in THP-1 cells exposed to LPS,and miR-302e mimics significantly decreased their production(P0.05).When treated with LPS,the monocytes from PBC patients produced significantly more IL-6 and TNF-α compared to those from healthy controls(P0.05).Conclusion Decreased miR-302e expression in monocytes of PBC patients may enhance IL-6 and TNF-α production and thus participate in the pathogenesis of PBC.
【Fund】: 国家自然科学基金(30972730 81072479 81170263);; 上海市科委基金(08QH14001 09JC1405400)~~
【CateGory Index】: R575.22
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【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 LI Xin-min1, MA Xiong2, QIU De-kai2. 1 Department of Gastroenterology, Fujian Provincial Hospital, Fuzhou 350001, China; 2 Renji Hospital, Shanghai Jiaotong University, Shanghai 200001, China;Clinical characteristics of patients with primary biliary cirrhosis[J];Modern Digestion & Intervention;2006-04
2 YAN Hui-ping, ZHUANG Hui, LIU Yan-min,FENG Xia, TAN Yu-fen, LIU Yan, ZHANG Xin, ZHAO Chun-hui. Beijing You An Hospital, Capital University of Medical Sciences, Beijing 100054, ChinaZHUANG Hui, Peking University Health Science Center, Beijing100070, China;Immunological characteristics of primary biliary cirrhosis patients[J];Chinese Journal of Hepatology;2005-01
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4 YAO Guangbi. Shanghai Jing An Qu Central Hospital, Shanghai 200040;A prospective study of primary biliary cirrhosis in Chinese patients[J];Chinese Hepatology;2002-03
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